畜牧与兽医2018，Vol.50Issue(6)：95-100,6.
华南地区猪圆环病毒3型感染的分子流行病学调查与分析
Prevalence of porcine circovirus 3 infection in South China
摘要
Abstract
Porcine circovirus type 3 (PCV3) is a novel emerging circovirus in recent years.The occurrence of PCV3 in South China remains unclear.In order to investigate the prevalence of PCV3 in South China,1 078 diseased samples were collected from 177 pig farms in Guangdong,Guangxi,Hainan,Fujian,Jiangxi and Hunan provinces through December 2016 to December 2017.PCR assay was conducted to detect the PCV3 genomic DNA.Positive samples were used to amplify and sequence the complete genome of PCV3.The PCR results demonstrated that the positive rate was 29.04％ (313/1078) at the sample level and 44.63％ (79/177) at the pig farm level.Complete genomic sequences were obtained from 10 PCV3 positive samples.The 10 complete genome sequences shared 98.8％ to 100％ nucleotide identity between each other and 98.2％-100％ nucleotide identity when compared with other PCV3 strains available in GenBank.Phylogenetic analysis based on PCV3 complete genomes showed that 37 PCV3 strains divided into two clusters.PCV3 strains CHN/HUNAN,CHN/GD-SH and CHN/GD-ZQ belonged to PCV3a clustering within US/M02016,whereas the other 7 strains in this study belonged to PCV3b.This study indicated that PCV3 had been widely spreading in South China and further research on epidemiology and pathology should be conducted to comprehensively understand PCV3.关键词
华南地区/猪圆环病毒/PCV3/分子流行病学/进化分析Key words
South China/porcine circovirus/PCV3/molecular epidemiology/phylogenetic analysis分类
农业科技引用本文复制引用
刘相聪,申翰钦,张鹏飞,黎嘉豪,刘燕玲,张乐宜,宋长绪..华南地区猪圆环病毒3型感染的分子流行病学调查与分析[J].畜牧与兽医,2018,50(6):95-100,6.基金项目
十二五农村领域国家科技计划项目(2015BAD12802-5) （2015BAD12802-5）
广东省现代农业产业共性技术创新团队项目(2016LM2150) （2016LM2150）
广州市科技计划项目(201508020062) （201508020062）
