刑事技术2018，Vol.43Issue(3)：210-214,5.DOI:10.16467/j.1008-3650.2018.03.008
SureID®PanGlobal试剂盒的法医学验证
Forensic Validation for the SureID? PanGlobal Kit
韩文明 1张庆霞 2王顺霞 2刘金杰 2赵怡2
作者信息
- 1. 北京市公安局大兴分局刑侦支队,北京 102612
- 2. 北京市公安司法鉴定中心,北京 100192
- 折叠
摘要
Abstract
Objective To evaluate the technical performance and forensic applicability of the SureID? PanGlobal Human DNA Identification kit. Methods In compliance with the GA/T 815-2009, the SureID? PanGlobal kit was verified in nine terms of sensitivity, accuracy, balance, species specificity, adaptability, consistency, stability, survivability and mixed samples. Results The SureID? PanGlobal kit meets the professional requirements with its internal standard and ladder, together with the accuracy of positive controls, the excellent detection sensitivity, the balance and the species specificity. All the results were correct with 10 repetitive freezing-thawing cycles of the kit. The endurance was shown by the kit against either the degraded or the inhibitor-spiked DNA samples under certain conditions. The genetic polymorphisms were successfully detected with a variety of diversely-disposed DNA samples, along with the consistency of their genotyping results. Conclusions All the tests demonstrated that SureID? PanGlobal Human DNA Identification kit is adaptive to the requirements for the human fluorescent STR multiplex PCR reagent. Since its technical level has reached up to that of the similar international products, the kit can be used in personal identification, paternity test and genetic analysis, suitable for handling actual criminal cases and DNA database building.关键词
法医物证学/短串联重复序列(STR)/SureID®PanGlobal人类DNA身份鉴定试剂盒Key words
forensic biological evidence/short tandem repeats (STR)/SureID® PanGlobal Human DNA Identification kit分类
社会科学引用本文复制引用
韩文明,张庆霞,王顺霞,刘金杰,赵怡..SureID®PanGlobal试剂盒的法医学验证[J].刑事技术,2018,43(3):210-214,5.
