中国人兽共患病学报2017，Vol.33Issue(8)：680-684,5.DOI:10.3969/j.issn.1002-2694.2017.08.003
猪链球菌2型转录调控因子Rex原核表达及体外结合活性分析
Prokaryotic expression of Rex transcription regulator in Streptococcus suis 2 and in vitro binding assay
摘要
Abstract
We cloned and prokaryoticly expressed the gene encoding Redox regulator(Rex)of Streptococcus suis serotype 2 and analyzed biological information and in vitro binding activity.The encoding Rex gene of SS2-1 strain was amplified by PCR with the designed primers,and then cloned into prokaryotic expression plasmid pET28a.The recombinant plasmid pET28a-Rex was transformed into E.coli BL21.After induced expression by IPTG,the Rex protein was obtained.The binding activity of Rex protein and DNA was analyzed by gel mobility shift assay (EMSA) in vitro.Purification of recombinant protein Rex was successfully expressed.The presence of NAD+ did not have major effect on mobility shift,but addition of NADH almost abolished such a binding activity.By in vitro binding assay,Rex was found to regulate the expression of Prex in response to NADH/NAD+ equilibrium.关键词
猪链球菌/Rex/调控因子Key words
Streptococcus suis/Rex/transcriptional regulator分类
医药卫生引用本文复制引用
王勇,祝昊丹,何孔旺,范红结..猪链球菌2型转录调控因子Rex原核表达及体外结合活性分析[J].中国人兽共患病学报,2017,33(8):680-684,5.基金项目
Supported by the National Genetically Modified Organisms Breeding Major Projects of China(No.2014ZX0800946B-002)and the Special Fund for Agro-scientific Research in the Public Interest(No.201303041)国家转基因生物新品种培育重大专项子课题(No.2014ZX0800946B-002) （No.2014ZX0800946B-002）
国家公益性行业科研专项(No.201303041) （No.201303041）
