中国人兽共患病学报2017，Vol.33Issue(8)：685-689,5.DOI:10.3969/j.issn.1002-2694.2017.08.004
一起腺病毒引起的聚集性呼吸道感染的实验室调查
Laboratory investigation of a clustered respiratory tract infection with human adenovirus
摘要
Abstract
In order to investigate the pathogens associated with a clustered event of fever occurred in a kindergarten in Fuzhou,Fujian Province,samples were collected from pediatric cases in the kindergarten and screened for various possible viral agents by real-time PCR.Of 10 respiratory specimens,7 were positive of human adenovirus (HAdV).The positive samples were inoculated into HEp-2 cell-lines for viral isolation.The PCR products of the hypervariable regions of Hexon gene were sequenced,followed by BLAST searches for viral type identification.In comparison with the strains prevalent in domestic or abroad in recent years,the deduced amino acid sequences showed no amino acid mutation in the hypervariable regions of Hexon.Combined with clinical manifestation and field epidemiological data,human adenovirus type 7 could be confirmed as the pathogen linked to the clustered event.关键词
人腺病毒/病毒分离/序列分析Key words
human adenovirus/viral isolation/sequence analysis分类
医药卫生引用本文复制引用
朱颖,林琦,游丽斌,黄枝妙,陈敏红,姚栩,张拥军,欧剑鸣,翁育伟..一起腺病毒引起的聚集性呼吸道感染的实验室调查[J].中国人兽共患病学报,2017,33(8):685-689,5.基金项目
Support by the Chinese National High Technology Research and Development Program (No.2011AA02A114)国家高技术研究计划(No.2011AA02A114) （No.2011AA02A114）
