中国人兽共患病学报2017，Vol.33Issue(8)：705-709,5.DOI:10.3969/j.issn.1002-2694.2017.08.008
沙门菌基质辅助激光解吸电离飞行时间质谱方法研究
Detecting Salmonella with matrix assisted laser desorption ionization time of flight mass spectrometry
摘要
Abstract
In order to optimize the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) method for Salmonella identification,study the target board pollution situation of different pretreatment methods,and establish a safe and effective method for detection of Salmonella,we applied MALDI-TOF-MS technique to detect 4 standard strains-of Salmonella under different culture conditions and sample preparation method,and established optimization program.MALDI target board was detected under different preparation treatments.The optimization method was used to detect 33 strains Salmonella which isolated from diarrhea patients' stool.Identification results were compared with serological results.The study found that MALDI-TOF-MS method could accurate identify of Salmonella in different pretreatment methods and culture medium.Thirty-three strains of Salmonella identified by MALDI-TOF-MS method were all accurate appraisal in genus level,19 strains of them were completely consistent with the serotyping identification results,14 strains of them were not consistent with the serotyping identification results.There was no bacteria growth on the target board in different pretreatment methods.MALDI-TOF-MS method can in a fast,convenient,safe and accurate way identify Salmonella,and it can become an effective means for identification of Salmonella.关键词
基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)/沙门菌/鉴定Key words
matrix-assisted laser desorption ionization time of flight mass spectrometry MALDI-TOF-MS)/Salmonella/identification分类
医药卫生引用本文复制引用
赵剑虹,高艳,李书明,张士尧,王恒伟,顾琳,韩庆华,焦洋,陈倩..沙门菌基质辅助激光解吸电离飞行时间质谱方法研究[J].中国人兽共患病学报,2017,33(8):705-709,5.基金项目
Supported by the Open Fund of Beijing(No.2015BZ0063-03)北京市开放基金(No.2015BZ0063-03) （No.2015BZ0063-03）
