中国人兽共患病学报2017，Vol.33Issue(8)：724-729,6.DOI:10.3969/j.issn.1002-2694.2017.08.012
基于COI基因片段的拟钉螺种类鉴定初探
Species identification of Triculinae based on COI gene segment, Fujian Province
摘要
Abstract
The aim of the study is to evaluate the utility value of species identification of Triculinae based on COI gene.Triculinae-like samples were collected from different places in Fujian Province.After classification based on morphology characters,genomic DNA was extracted from collected samples.Then the segments of COI gene were amplified and sequenced for taxonomy annotation and phylogenetic analysis.Eleven samples were annotated as Gammatricula (identities:88.96％-97.82％),the other two were annotated as Tricula wumingensis (identity:87.08％) and Neotricula apart (identity:88.55％),respectively.In most cases (12 out of 13),there was a difference between results based on different classification methods on a genus level.The alignment of COI gene segment is sufficient for preliminary identification of Triclulinae at family level.However,it is need further study in species identification of Triclulinae at genus level.关键词
拟钉螺亚科/COI基因/物种鉴定Key words
Triclulinae/COI/species classification phylogenetic分类
医药卫生引用本文复制引用
柯雪梅,邱旻,林陈鑫,葛婧,郭志南,程明基,陈清,陈国伟..基于COI基因片段的拟钉螺种类鉴定初探[J].中国人兽共患病学报,2017,33(8):724-729,6.基金项目
Supported by the Science and Technology Agency og Fujian Province (No.2011D015)福建省科技厅项目(No.2011D015) （No.2011D015）
