中国人兽共患病学报2017，Vol.33Issue(8)：744-747,752,5.DOI:10.3969/j.issn.1002-2694.2017.08.016
结核分枝杆菌rBCG-Rv2029c重组疫苗的构建与鉴定
Construction and identification of recombinant rBCG-Rv2029c vaccine of Mycobacterium tuberculosis
摘要
Abstract
We constructed a recombinant vaccine of Mycobacterium tuberculosis rBCG-Rv2029c,and then identified it.Rv2029c antigen encoding gene was amplified by PCR.The enzyme digestion products were ligated into rpMV261-2029c recombinant plasmid,after double digestion of Rv2029c and pmv261 vector,and then we introduced the plasmid into BCG to construct rBCG-Rv2029c recombinant vaccine by electroporation method.Finally,we analyzed the expression of the recombinant protein by SDS-PAGE and Western blotting.A total of 1 020 bp Rv2029c gene successfully amplified by PCR was inserted into the plasmid pmv261,then the fusion gene was successfully transduced into BCG.After identified by double enzyme digestion,confirmed by gene alignment and by thermally induced with Western blotting,the recombinant protein had a free primary.The recombinant live vaccine of M.tuberculosis rBCG-Rv2029c is successfully constructed,which lay a foundation for the study of the immune mechanism of recombinant vaccine.关键词
卡介苗/重组疫苗/结核杆菌/Rv2029cKey words
BCG vaccine/recombinant vaccine/Mycobacterium tuberculosis/Rv2029c分类
医药卫生引用本文复制引用
薛士鹏,吴建勇,宋彬,齐红双,李英,党颍徐,满永宏..结核分枝杆菌rBCG-Rv2029c重组疫苗的构建与鉴定[J].中国人兽共患病学报,2017,33(8):744-747,752,5.基金项目
Supported by the Natural Science Foundation of Nanyang Medical College (No.NYYZ006)校自科NYYZ006 （No.NYYZ006）
