中国现代医学杂志2017，Vol.27Issue(22)：1-6,6.DOI:10.3969/j.issn.1005-8982.2017.22.001
复合羟固醇无机小牛骨块修复犬下颌骨临界骨缺损的组织学研究
Oxysterols coated bovine bone blocks used in critical-sized defects of mandible: a histomorphometric study in dogs
摘要
Abstract
Objective To compare osteogenic effect of oxysterols coated bovine bone blocks (OBB) and bovine bone particles (BBP) in critical-sized dog mandible defects at the early stage of bone healing.Methods Four Beagle dogs were selected.Following extraction of the third and fourth mandibular premolars as well as the first molar (P3-M1),two standardized box-shaped defects (5×l0×l0 mm) were created at the alveolar ridge in each quadrant (4 defects per animal,a total of 16).After 4 weeks of healing period,the defects were reshaped and randomly allocated in a split-mouth design to either OBB (8 defects) or BBP (8 defects) group.All animals were euthanized after 4 weeks.The specimens were dehydrated for hematoxylin and eosin staining and non-decalcified sectioning.Results The postoperative healing was good.OBB exhibited abundant osteoblasts and new bone formation in the defect area.Histomorphometrical analysis (HE staining) revealed that the percentage of new bone was (37.22 ± 2.16)％ in the OBB group and (34.70 ± 2.66)％ in the BBP group,there was no significant difference between the two groups (P ＞0.05);and the number of osteoblasts was (31.13 ± 2.85) in the OBB group which was significantly larger than (24.88 ±2.95) in the BBP group (P ＜ 0.05).Histomorphometrical analysis (methylene blue-acidic fuchsin staining) revealed that the rate of new bone formation was (20.13 ± 1.32)％ in the OBB group and (19.28 ± 0.82)％ in the BBP group,the degradation rate was (30.80 ± 1.16)％ in the OBB group and (29.69 ± 1.12)％ in the BBP group,the differences were not statistically significant (P ＞ 0.05).Conclusions OBB have similar osteogenic effect as BBP at the early stage of bone healing.Because OBB have good space maintaining property,they are more suitable for the repair of large bone defects.关键词
小牛骨/骨诱导性/成骨细胞/骨缺损Key words
bovine bone/osteoinductivity/osteoblast/bone defect分类
医药卫生引用本文复制引用
胡晓,刘威,吴王喜,桂媛媛,周磊..复合羟固醇无机小牛骨块修复犬下颌骨临界骨缺损的组织学研究[J].中国现代医学杂志,2017,27(22):1-6,6.基金项目
国家自然科学基金(No:81170998) （No:81170998）
广东省医学科研基金(No:C2012034) （No:C2012034）
