中国现代医学杂志2017，Vol.27Issue(22)：36-41,6.DOI:10.3969/j.issn.1005-8982.2017.22.007
MicroRNA-25表达下调对神经胶质瘤U87细胞增殖与凋亡的影响
Effect of down-regulation of miR-25 expression on proliferation and apoptosis of glioma U87 cells
庞智寅 1张云鹤 1张涛 2杨颖 2付爱军 1李建民 1杨群福2
作者信息
- 1. 华北理工大学附属医院神经外科,河北唐山063000
- 2. 河北医科大学附属邢台市人民医院神经外科,河北邢台054000
- 折叠
摘要
Abstract
Objective To study the effect of down-regulation of miR-25 expression on the proliferation and apoptosis of glioma U87 cells,and the regulatory effect of miR-25 and its target gene Bcl-2 on the proliferation and apoptosis process of glioma cells.Methods U87 cells were transfected by lentiviral-mediated antisense miR-25 oligonucleotides.In the experiment groups (transfected with anti-miR-25),control groups (transfected with negative control) and blank groups (blank PBS),RT-PCR was used to detect the mRNA expression levels of miR-25 and Bcl-2,CCK method was used to detect cell proliferation,flow cytometry was used to detect the cell cycle and apoptosis.Results The mRNA expression levels of miR-25 and Bcl-2 in the experiment groups were lower than those in the the blank groups and the control groups,the differences were statistically significant (P ＜ 0.05).Compared with the blank groups and the control groups,the cell proliferation activity was decreased,the cell cycle was delayed and the apoptosis rate was increased in the experiment groups.Conclusions In glioma U87 cells,down-regulation of miR-25 expression delays cell cycle,inhibits cell proliferation,and promotes apoptosis by targeting Bcl-2 gene.关键词
神经胶质瘤/microRNA-25/B细胞淋巴瘤/白血病-2基因/增殖/凋亡Key words
glioma/miR-25/Bcl-2/proliferation/apoptosis分类
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庞智寅,张云鹤,张涛,杨颖,付爱军,李建民,杨群福..MicroRNA-25表达下调对神经胶质瘤U87细胞增殖与凋亡的影响[J].中国现代医学杂志,2017,27(22):36-41,6.
