中国现代医学杂志2018，Vol.28Issue(17)：1-5,5.DOI:10.3969/j.issn.1005-8982.2018.17.001
酸性环境抑制高磷诱导大鼠VSMCs钙化的机制研究
Mechanisms of acidic environment inhibiting vascular smooth muscle cell calcification induced by high phosphorus in rats
摘要
Abstract
Objective To explore the effect and possible mechanisms of acidification on high phosphorus-induced vascular smooth muscle cell (VSMC) calcification in rats. Methods VSMCs were isolated from rat aorta, and randomly divided into control group and high phosphorus group. The high phosphorus group was further settled into two subgroups, i.e. high phosphorus+pH 7.1 group and high phosphorus+pH 7.4 group, which were treated with β-glycerophosphate, and acidified by HCl to adjust the pH. The mRNA expressions of nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) and Runt-related transcription factor 2 (RUNX2) in VSMCs were detected by RT-PCR after stimulation for 4 days. The activity of alkaline phosphatase (ALP) and calcium deposition were tested after culture for 14 days. Results Compared to the control group, calcium depositon and ALP activity were significantly increased in the high phosphorus+pH 7.4 group (P < 0.05), and the expressions of NFATc1 and RUNX2 were upregulated as well (P < 0.05). Meanwhile, the calcification level and the expressions of RUNX2 and NFATc1 in the high phosphorus+pH 7.1 group were lower than those in the high phosphorus+pH 7.4 group (P < 0.05). NFATc1 expression level was positively correlated with ALP activity and RUNX2 expression level (P < 0.05). Conclusions Acidic environment can inhibit VSMC calcification induced by high phosphorus in rats, itsmechanism is possibly by downregulation ofNFATc1 expression and prevention of VSMC phenotypic transformation.关键词
血管钙化/血管平滑肌细胞/活化T细胞转录因子c1/Runt相关转录因子2/慢性肾衰竭Key words
vascular calcification/vascular smooth muscle cell/NFATc1/RUNX2/chronic renal failure分类
医药卫生引用本文复制引用
耿同会,徐金升,韩迎迎,白亚玲,张俊霞,崔立文,张胜雷..酸性环境抑制高磷诱导大鼠VSMCs钙化的机制研究[J].中国现代医学杂志,2018,28(17):1-5,5.基金项目
河北省自然科学基金(No:H2012206157) （No:H2012206157）
河北省科技计划项目(No:16397733D) （No:16397733D）
