中国现代医学杂志2018，Vol.28Issue(17)：13-19,7.DOI:10.3969/j.issn.1005-8982.2018.17.003
小鼠骨髓间充质干细胞自噬与衰老的关系
Correlation between autophagy of bone marrow mesenchymal stem cells and aging in mice
孙志敏 1张铮 2文童 2张丽娟 2杨超 3柳彦君 2胡桃红2
作者信息
- 1. 锦州医科大学火箭军总医院研究生培养基地,辽宁 锦州 121000
- 2. 中国人民解放军火箭军总医院 心血管内科,北京 100088
- 3. 中国人民解放军火箭军总医院输血科,北京 100088
- 折叠
摘要
Abstract
Objective To investigate the effect of autophagy of bone marrow mesenchymal stem cells (BM-MSCs) on the aging process in mice. Methods BM-MSCs were isolated from the bone marrow of young (8 w) and elderly (18 m) mice respectively. BM-MSCs were cultivated in vitro, and characterized using flow cytometry. Apoptosis was detected by TUNEL staining. Meanwhile, the expressions of VEGF, bFGF, IGF-1 and HGF in the BM-MSCs were detected by ELISA kits. The expressions of autophagy-related proteins (such as LC3-Ⅰ, LC3-Ⅱ, Beclin 1, ATG12-5 and p62) and signal pathway proteins (such as p-Akt, Akt, p-mTOR and mTOR) were evaluated by Western blot. Results Compared with the young group, the apoptosis in the aged group was significantly increased (P < 0.05), while the secretory function was decreased (P < 0.05). Furthermore, the expressions of LC3-Ⅰ, LC3-Ⅱ, Beclin 1 and ATG12-5 were decreased (P < 0.05), whereas p62 was increased (P < 0.05) in the aged group. The expressions of p-Akt, Akt, p-mTOR and mTOR were increased in the aged group. Conclusions In aged mice, apoptosis of BM-MSCs increases, paracrine function and autophagy activity decrease.关键词
骨髓间充质干细胞/自噬/衰老Key words
mesenchymal stem cell (MSC)/autophagy/aging分类
医药卫生引用本文复制引用
孙志敏,张铮,文童,张丽娟,杨超,柳彦君,胡桃红..小鼠骨髓间充质干细胞自噬与衰老的关系[J].中国现代医学杂志,2018,28(17):13-19,7.
