中国现代医学杂志2018，Vol.28Issue(17)：26-30,5.DOI:10.3969/j.issn.1005-8982.2018.17.005
α-突触核蛋白对小鼠星形胶质细胞的激活作用
Alpha synuclein activates astrocytes in mice
摘要
Abstract
Objective To investigate the activation effect of α-synuclein (α-syn) on astrocytes and innate immunity. Methods Primary astrocytes of mice were isolated and treated with α-syn (wild type, Wt) and its A53T mutant. Quantitative real-time PCR (qRT-PCR) and Western blot analysis were used to examine altered expressions of toll-like receptors (TLRs) 1-4 and NF-κB. Results In comparison with the control groups, Wt α-syn and its A53T mutant increased mRNA expressions of TLRs 1-4 and NF-κB, displaying a statistical significance (P < 0.05), with the A53T mutant having more potent immune induction effect than Wt α-syn. Western blot analysis showed the A53T mutant upregulated protein expressions of TLR2 and NF-κB. Conclusions Alpha synuclein is a powerful immunogenic substance to activate the astrocyte-mediated innate immune response, which provides a strong immunological evidence for the pathogenesis of neurodegenerative diseases.关键词
α-突触核蛋白/星形胶质细胞/Toll样受体/神经退行性疾病Key words
α-synuclein/astrocyte/toll-like receptors/neurodegenerative diseases分类
医药卫生引用本文复制引用
王进堂,Jeremy Walston,Peisong Gao,高茂龙,Sean X Leng,陈峥..α-突触核蛋白对小鼠星形胶质细胞的激活作用[J].中国现代医学杂志,2018,28(17):26-30,5.基金项目
美国米尔斯坦亚美医学基金会奖学金项目 （）
