中国现代医学杂志2018，Vol.28Issue(17)：31-36,6.DOI:10.3969/j.issn.1005-8982.2018.17.006
甲基莲心碱在氧化应激中的作用机制研究
Mechanism of neferine in antioxidant stress
刘向东 1李慧 2王成志 3赵焕东 3肖平1
作者信息
- 1. 中南大学湘雅医院 肾内科,湖南 长沙 410008
- 2. 湖南省长沙市第一医院 呼吸科,湖南 长沙 410005
- 3. 中南大学湘雅医院 卫生部纳米生物技术重点实验室,湖南 长沙 410007
- 折叠
摘要
Abstract
Objective To investigate the effect of neferine (Nef) on oxidative stress and its mechanism. Methods NRK-52E cells (normal rat kidney proximal tubular epithelial cells) were cultured in vitro and divided into six groups: control group, Mannitol group, high-glucose group (HG), HG+Nef group, zinc protoporphyrin Ⅸ group (ZnP) and HG+Nef+ZnP group. Cell viability was detected by CCK-8. The expressions of heme oxygenase-1 (HO-1) and p67 were detected by Western blot. Reactive oxygen species (ROS) was detected by DCFH-DA probes. The level of malondialdehyde (MDA) was measured by TBA and the activity of HO-1 was measured by TBIL. Results Compared with the control group, the oxidative stress indexes p67, ROS and MDA were increased, but the cell viability was decreased in the HG group (P < 0.05). Compared with the HG group, the expression and activity of HO-1 and cell viability were increased, but the expression of p67 and the levels of ROS and MDA were decreased in the HG+Nef group (P < 0.05). The effect of neferine mentioned above was reduced in the HG+Nef+ZnP group, compared with the HG+Nef group (P < 0.05). Conclusions Neferine attenuates oxidative stress injury in NRK-52E cells by inducing HO-1 expression and increasing its enzymatic activity.关键词
甲基莲心碱/血红素加氧酶1/大鼠近端肾小管上皮细胞/氧化应激Key words
neferine/heme oxygenase 1/NRK-52E/reactive oxygen species分类
医药卫生引用本文复制引用
刘向东,李慧,王成志,赵焕东,肖平..甲基莲心碱在氧化应激中的作用机制研究[J].中国现代医学杂志,2018,28(17):31-36,6.
