中国循证儿科杂志2017，Vol.12Issue(1)：7-11,5.DOI:10.3969/j.issn.1673-5501.2017.01.002
组蛋白去乙酰化酶7基因表达与儿童急性淋巴细胞白血病临床生物学特征及预后的相关性
Association of histone deacetylase 7 expression and the clinico-biological characteristics and outcome in childhood acute lymphoblastic leukemia
摘要
Abstract
Objective To discuss the expression of histone deacetylase 7 (HDAC7) in bone marrow samples of patients with newly diagnosed pediatric acute lymphoblastic leukemia (ALL) and the association with clinic-biological characteristics,early treatment responses and long-term outcome.Methods The HDAC7 expression level of pediatric patients with newly diagnosed ALL was determined by RT-qPCR method.Three samples from ALL patients in CCR for more than 3 years after treatment were used as control and patients were divided into high-expression (≥ 1.0) and low-expression (＜ 1.0) groups.The differences of clinicobiological characteristics,minimal residual disease and event-free survival (EFS) were analyzed between high and low expression groups.Results HDAC7 expression of 236 pediatric patients with newly diagnosed ALL ranged from 0.046 to 10.581,with 124 and 112 patients in the high expression group and low expression group,respectively.Univariate analysis showed that the expression of HDAC7 was associated with low peripheral white blood count (WBC) at diagnosis(＜50× 109 · L-1),pro-B immunophenotype and MLL gene rearrangement(all P＜0.05).Logistic multivariate regression analysis showed that WBC (＜50× 109 · L-1) and fusion gene were influence factors of HDAC7 expression(all P＜0.05).Intermediate risk group patients with high-expression of HDAC7 was associated with a favorable 5 year EFS compared with low-expression group,with(91.0±3.5)％ vs.(75.5±4.9)％,P=0.013.Furthermore,HDAC7 expression was indentified as the independent prognostic factor for EFS in IR patients,with odd ratio and 95％ confidence interval of 1.26(1.31～9.51).Concluslon In pediatric ALL,expression of HDAC7 was found to be related to clinical and biological characteristics.Low-HDAC7 was independent adverse prognostic factor for EFS in IR subgroup.关键词
急性淋巴细胞白血病/儿童/组蛋白去乙酰化酶7/预后/基因表达Key words
Acute lymphoblastic leukemia/Child/Histone deacetylase 7/Prognosis/Gene expression引用本文复制引用
侯贝,高超,刘曙光,张瑞东,陆继冉,郑胡镛..组蛋白去乙酰化酶7基因表达与儿童急性淋巴细胞白血病临床生物学特征及预后的相关性[J].中国循证儿科杂志,2017,12(1):7-11,5.基金项目
国家自然科学81300432,81300434 （）
北京市医院管理局重点医学专业发展计划项目“扬帆计划”临床医学发展专项:ZY201404 （）
北京市医院管理局“登峰”人才培养计划:DFL20151101 （）
首都卫生发展科研专项-重点攻关项目:2016-1-2091 （）
