中国岩溶2018,Vol.37Issue(1):74-80,7.DOI:10.11932/karst2017y58
岩溶与非岩溶区稻田土壤CO2固定细菌的丰度比较
Comparison of abundance of CO2 fixing microbes in paddy soil from karst and non-karst area
摘要
Abstract
oil samples were collected from paddy field in the Maocun karst experimental site to study the gene abundance of cbbLR1,cbbLG1 and cbbM,as the index of CO2 fixing bacteria in typical karst area,mixed area and non-karst area with the utilization of real-time quantitative PCR technology.The results showed that the gene abundances of cbbLG1 in karst area were generally higher than that in non-karst area,and the maximum value was 1.42× 109copies · g-1.The gene abundances of cbbLR1 and cbbM in mixed area were significantly higher than that in non-karst area,and the maximum values were 2.06 × 109 copies · g-1 and 3.35 × 107 copies · g-1.Correlation analysis showed that the abundance of cbbLG1 was significantly correlated with organic carbon mass fraction,total nitrogen mass fraction,and cation exchange capacity in the soil,which were significant factors that affect the gene abundance of cbbLG1.The abundance of the three cbbL genes had different sensitivity to pH,which was significantly positive correlated with the cbbLG1 gene and negatively correlated with the cbbM gene.关键词
岩溶区/水稻土/土壤性质/cbbL基因Key words
karst area/paddy soil/soil properties/cbbL gene分类
农业科技引用本文复制引用
王腾,靳振江,郭家怡,张双双,程亚平,张琴,李金城..岩溶与非岩溶区稻田土壤CO2固定细菌的丰度比较[J].中国岩溶,2018,37(1):74-80,7.基金项目
国家自然科学基金项目(41361054,41003038) (41361054,41003038)
广西自然科学基金项目(2011GXNSFD018002,2011GXNSFA018006,2010GXNSFB013004 ()
桂科合14123001-13) ()
岩溶动力学重点实验室开放基金项目(KDL2010-02,KDL2011-10) (KDL2010-02,KDL2011-10)
广西矿冶与环境科学实验中心项目资助(KH2012ZD004) (KH2012ZD004)
广西高等学校立项科研项目(201204LX162,201106LX229) (201204LX162,201106LX229)
广西高等学校高水平创新团队及卓越学者计划项目(002401013001) (002401013001)
桂林理工大学博士启动基金(2004041) (2004041)