中国岩溶2018，Vol.37Issue(1)：81-90,10.DOI:10.11932/karst20180104
小球藻对岩溶水体Ca2+、HCO3-利用效率实验研究
Experimental study on the utilization efficiency of Chlorella to Ca2+ and HCO3-in karst water
摘要
Abstract
Chlorella was collected from Dalongdong cave in Shanglin county of Guangxi to study its utilization efficiency of Ca2+ and HCO3-in three water bodies with different concentrations of DIC (4.6 mmol · L-1,2.5 mmol· L-1 and0.5 mmol· L-1) in the closed system.The results show that:(1)After7 days of cultivation in three different DIC solution of 4.6 mmol · L-1、2.5 mmol · L-1 and 0.5 mmol · L-1,Chlorella biomass increased from 0.04 Abs,to 0.56 Abs,0.50 Abs and 0.44 Abs,respectively.In the environment with high HCO3-and Ca2+ concentration,Chlorella absorbed 54.95％ of HCO3-in group A and 48.00％ of HCO3-in group B,it also absorbed 28.26％ of Ca2+ in group A and 24.14％ of Ca2+ in group B.In the environment with low HCO3-concentration,it was difficult for Chlorella to absorb HCO3-in group C (0.5 mmol · L-1).These findings indicate that the high concentration of HCO3-in karst reservoirs plays a role of "fertilization" on the growth of Chlorella,which is significant to the karst process-related carbon sink;(2)The amount of Ca2+ deposition caused by Chlorella photosynthesis using HCO3-was higher than the amount of Ca2+ absorbed by Chlorella photosynthesis;(3)Photosynthesis from Chlorella make δ13CDIC positive,while the respiration make δ13CDIC negative in the culture medium.关键词
岩溶水库/小球藻/碳汇/δ13CDIC/碳酸钙沉积Key words
karst reservoir/Chlorella/carbon sink/δ13CDIC/Calcium carbonate precipitation分类
天文与地球科学引用本文复制引用
张陶,李建鸿,蒲俊兵,李瑞,吴飞红,李丽..小球藻对岩溶水体Ca2+、HCO3-利用效率实验研究[J].中国岩溶,2018,37(1):81-90,10.基金项目
中央高校基本科研业务费专项(XDJK2017D024) （XDJK2017D024）
中国地质科学院基本科研业务费项目(YYWF201636) （YYWF201636）
广西自然科学基金项目(2016GXNSFCA380002,2017GXNSFFA198006) （2016GXNSFCA380002,2017GXNSFFA198006）
国家自然科学基金项目(41572234,41702271) （41572234,41702271）
中国地质调查局地质调查项目(DD20160305-03)和岩溶所基本科研业务费资助项目(2017006) （DD20160305-03）
