中华临床免疫和变态反应杂志2018，Vol.12Issue(2)：156-163,8.DOI:10.3969∕j.issn.1673-8705.2018.02.004
嗜酸性粒细胞性胃肠炎儿童胃黏膜血管活性肠肽及其受体表达与嗜酸性粒细胞数量及总IgE水平变化的关系
Correlations between vasoactive intestinal peptide and its receptor expression in gastric mucosa with amount of eosinophils and total IgE levels among children with eosinophilic gastroenteritis
钱淑怡 1杨辉 1金玉 1刘志峰 1朱航 1吴润秋 1练敏1
作者信息
- 1. 210008,南京医科大学附属儿童医院消化科
- 折叠
摘要
Abstract
Objective To investigate the relationship among vasoactive intestinal peptide ( VIP ) and vasoactive intestinal peptide type 2 receptor ( VPAC2) expression in gastric mucosa with amount of eosinophils ( EOS) and total immunoglobulin E ( IgE) levels in children with eosinophilic gastroenteritis ( EG) . Methods Twenty children who were diagnosed as EG were collected from the Department of Gastroenterology, Children's Hospital Affiliated to Nanjing Medical University and enrolled in EG group from July 2015 to August 2016, with the male 16 and female 4, and the average age was (6. 34±4. 51) years-old in the EG group. Another 20 children with normal biopsy results of gastrointestinal mucosa tissue were enrolled in the control group, with the male 15 and female 5, and the average age was (5. 46±3. 27) years-old in the control group. Amount of EOS and total IgE level of peripheral blood were detected. The amount of EOS in gastric mucosa was counted by HE staining. The expression of VIP and VPAC2 in gastric mucosa was detected by using immunohistochemistry. The relationship among the content of VIP and VPAC2 in gastric mucosa, amount of EOS in gastric mucosa and total IgE level in peripheral blood were analyzed. Results Amount of EOS and total level of IgE in peripheral blood were 1. 64 ( 0. 06, 27. 37) × 109/L and 477. 7 ( 173. 7, 4437. 0) U/ml in the EG group, respectively, which were significantly higher than 0. 13 (0. 04, 0. 85) ×109/L and 55. 9 ( 21. 9, 97. 3) U/ml in the control group ( both at P<0. 05) . The contents of VIP and VPAC2 in gastric mucosa in the EG group were signifi-cantly lower than those in the control group ( both at P<0. 05) . Negative correlations were showed between the content of VIP with the EOS amount in gastric mucosa and total level of IgE in peripheral blood ( r=-0. 81,-0. 83, both at P<0. 01) , and negative correlations also were found between VPAC2 with the EOS amount in gastric mucosa and total level of IgE in peripheral blood ( r=-0. 72, -0. 77, both at P<0. 05) . Conclusions The amount of EOS and total IgE level in peripheral blood in the EG group are increased and the amount of EOS in gastric mucosa is also increased in comparison to the control group and shows negative correlations with VIP and VPAC2 expression, indicating that VIP probably participats in pathogenesis of EG.关键词
胃肠炎/嗜酸性粒细胞/血管活性肠肽/血管活性肠肽2型受体/儿童Key words
Gastroenteritis/Eosinophi/Vasoactive intestinal peptide/Vasoactive intestinal peptide re-ceptor-2/Children引用本文复制引用
钱淑怡,杨辉,金玉,刘志峰,朱航,吴润秋,练敏..嗜酸性粒细胞性胃肠炎儿童胃黏膜血管活性肠肽及其受体表达与嗜酸性粒细胞数量及总IgE水平变化的关系[J].中华临床免疫和变态反应杂志,2018,12(2):156-163,8.
