中成药2017，Vol.39Issue(1)：55-60,6.DOI:10.3969/j.issn.1001-1528.2017.01.010
苦参碱纳米粒及其麦胚凝集素修饰产物的制备
Preparation of matrine nanoparticles and their wheat germ agglutinin-modified product
摘要
Abstract
AIM To prepare the matrine nanoparticles and their wheat germ agglutinin-modified product.METHODS Double emulsification-solvent evaporation method was employed to prepare matrine nanoparticles.In consideration of influencing factors of ratio of poly (lactic-co-glycolic acid) to matrine,rotational speed and polyvinyl alcohol concentration,as well as the evaluation indices of particle size,potential,encapsulation efficiency and drug load,the preparation was optimized by central composite design.Wheat germ agglutinin-modified matrine nanoparticles were prepared by carbodiimide method.In addition to the influencing factors of ratio of carbodiimide to N-hydroxysuccinimide,wheat germ agglutinin addition and incubation time,evaluation indices of particle size,potential and modification rate were also taken into account in the preparation optimization by uniform design.RESULTS The optimal conditions for matrine nanoparticles were determined to be 0.594 ∶ 1 for ratio of poly (lactic-co-glycolic acid) to matrine,815 r/min for rotational speed,and 0.46％ for polyvinyl alcohol concentration.The average particle size,potential,encapsulation efficiency and drug loading were 112.04 nm,-15.38 mV,90.05％ and 27.14％,respectively.The optimal conditions for their wheat germ agglutinin-modified product were found to be 2.8 ∶ 0.12 for ratio of carbodiimide to N-hydroxysuccinimide,3 mg for wheat germ agglutinin consumption,and 14 h for incubation time.The average particle size,potential and modification rate were 474.7 nm,-5.2 mV and 69.51％,respectively.CONCLUSION The preparation techniques are reliable,and the matrine nanoparticles and their wheat germ agglutinin-modified product show their stable properties.关键词
苦参碱/纳米粒/麦胚凝集素/复乳化-溶剂挥发法/碳二亚胺法/星点设计/均匀设计Key words
matrine/nanoparticles/wheat germ agglutinin/double emulsification-solvent evaporation method/carbodiimide method/central composite design/uniform design分类
医药卫生引用本文复制引用
段晓颖,贾庆涵,刘丽娜,程远方..苦参碱纳米粒及其麦胚凝集素修饰产物的制备[J].中成药,2017,39(1):55-60,6.基金项目
河南省科技攻关项目(132102310267) （132102310267）
