中成药2017，Vol.39Issue(1)：70-75,6.DOI:10.3969/j.issn.1001-1528.2017.01.013
星点设计-响应面法优化黄芪双水相萃取工艺
Optimization of two-phase aqueous extraction for Astragali Radix by central composite design-response surface method
摘要
Abstract
AIM To optimize the two-phase (ethanol-dipotassium phosphate) aqueous extraction for Astragali Radix by central composite design-response surface method.METHODS With ethanol concentration,dipotassium hydrogen phosphate solution concentration and Astragali Radix feed ratio as influencing factors,together with extraction rates of total flavonoids and total saponins as evaluation indices,the technology optimization was accomplished by central composite design-response surface method.RESULTS The optimal conditions were determined to be 27.97％ for ethanol concentration,22.03％ for dipotassium hydrogen phosphate solution concentration,and 1 ∶ 58.85 for Astragali Radix feed ratio.The extraction rate of total flavonoids reached 74.13％ (concentrated in the upper layer),while that of total saponins reached 81.34％ (concentrated in the lower layer).CONCLUSION With good predictability,this simple and accurate method can be used for the extraction of total flavonoids and total saponins in Astragali Radix.关键词
黄芪/总黄酮/总皂苷/双水相萃取/星点设计-响应面法Key words
Astragali Radix/total flavonoids/total saponins/aqueous two-phase extraction/central composite design and response surface method分类
医药卫生引用本文复制引用
宋力飞,刘常青,李曼莎,段海霞,马志鹏,刘乡乡..星点设计-响应面法优化黄芪双水相萃取工艺[J].中成药,2017,39(1):70-75,6.基金项目
国家高技术研究发展计划(2014AA022204) （2014AA022204）
