中成药2017，Vol.39Issue(1)：98-101,4.DOI:10.3969/j.issn.1001-1528.2017.01.019
HPLC法同时测定养心定悸胶囊中4种成分
Simultaneous determination of four constituents in Yangxin Dingji Capsules by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Yangxin Dingji Capsules (a cardiac tonic for palpitation,containing Glycyrrhizae Radix et Rhizoma Praeparata cum Melle,Cinnamomi Ramulus,Rehmanniae Radix,etc.).METHODS The analysis of 50％ methanol extract of Yangxin Dingji Capsules was performed on a 40 ℃ thermostatic Diamonsil C1s column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of 0.1％ formic acid-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 265 nm.RESULTS Liquiritin,glycyrrhizic acid,cinnamic acid and cinnamic aldehyde showed good linear relationships within the ranges of 1.00-80.24 μg/mL (r=0.999 0),2.52-100.70 μg/mL (r--0.999 7),0.50-40.40 μg/mL (r =1.000 0) and 0.66-52.96 μg/mL (r =1.000 0),whose average recoveries were 97.74％,100.97％,101.48％ and 99.49％ with the RSDs of 0.45％,1.11％,1.27％ and 1.66％,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Yangxin Dingji Capsules.关键词
养心定悸胶囊/甘草苷/甘草酸/桂皮酸/桂皮醛/HPLCKey words
Yangxin Dingji Capsules/liquiritin/glycyrrhizic acid/cinnamic acid/cinnamic aldehyde/HPLC分类
医药卫生引用本文复制引用
王晗雪,李林丰,安叡,程雪梅,王长虹,王新宏..HPLC法同时测定养心定悸胶囊中4种成分[J].中成药,2017,39(1):98-101,4.基金项目
上海市国内科技合作领域项目(14495800200) （14495800200）
