中成药2017，Vol.39Issue(1)：116-120,5.DOI:10.3969/j.issn.1001-1528.2017.01.023
制何首乌3种成分在动脉粥样硬化大鼠血浆中的测定
Determination of three constituents in Polygoni multiflori Radix Praeparata in plasma of atherosclerosis rats
摘要
Abstract
AIM To establish an HPLC method for determining the contents of three constituents in Polygoni multiflori Radix Praeparata in plasma of atherosclerosis rats.METHODS After the rats were intragastrically administrated with Polygoni multiflori Radix Praeparata CMC-Na solution,the plasma was collected.The HPLC analysis was carried out on a 30 ℃ thermostatic Hypersil C1s column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.03％ phosphoric acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.DAS2.0 software was applied to drawing concentration-time curves and calculating pharmacokinetic parameters.RESULTS Stilbene glucoside,emodin and physcion showed good linear relationships within the ranges of 61.25-6 125 μg/L (r =0.999 8),12.6-3 150 μg/L (r =0.999 3) and 24.1-6 030 μg/L (r =0.999 5),respectively.The method recoveries were 99.5％-105.8％ with the RSDs of 1.3％-3.3％,while the extraction recoveries were 87.2％-96.3％ with the RSDs of 3.2％-5.9％.The pharmacokinetic behaviors of three constituents all accorded with two-compartment model,but their contents in plasma were much lower than those in medicinal material.CONCLUSION The bioavailabilities of stilbene glucoside,emodin and physcion are relatively low in plasma of atherosclerosis rats,which may be related to constituents' intestinal absorption after intragastric administration with Polygoni multiflori Radix Praeparata.关键词
制何首乌/二苯乙烯苷/大黄素/大黄素甲醚/药动学行为/动脉粥样硬化大鼠/血浆/HPLCKey words
Polygoni multiflori Radix Praeparata/stilbene glucoside/emodin/physcion/pharmacokinetic behaviors/atherosclerosis rats/plasma/HPLC分类
医药卫生引用本文复制引用
左雅敏,伍庆,李兴,潘美秀,刘国旗,潘卫..制何首乌3种成分在动脉粥样硬化大鼠血浆中的测定[J].中成药,2017,39(1):116-120,5.基金项目
国家自然科学基金资助项目(81560720) （81560720）
贵阳市科技局2013社会发展与民生科技计划(筑科合同[2013103] 20) （筑科合同[2013103] 20）
教育部喀斯特山地生物多样性保护与可持续利用创新团队(IRT1227) （IRT1227）
喀斯特地区生物与信息技术协同创新中心(黔教合协同创新字[2014]04) （黔教合协同创新字[2014]04）
贵州省科技成果转化引导基金计划项目(黔科合成转字[2015] 2015-2) （黔科合成转字[2015] 2015-2）
