中成药2017，Vol.39Issue(1)：121-125,5.DOI:10.3969/j.issn.1001-1528.2017.01.024
青天葵4种提取物抗氧化活性与其总黄酮和总酚含量的相关性
Correlations between antioxidant activities and contents of total flavonoids and total phenols of four extracts from Nervilia fordii
摘要
Abstract
AIM To investigate the correlations between antioxidant activities and contents of total flavonoids and total phenols of chloroform,ethyl acetate,n-butanol and ethanol extracts from Nervilia fordii (Hance) Schltr..METHODS The contents of total flavonoids and total phenols in four extracts from N.fordii were determined by AlCl3-HAc-NaAc coloration method and Folin-Ciocalteu colorimetric method,respectively.The antioxidant activities of extracts were evaluated by determining scavenging activities of DPPH radical,hydroxyl radical and ABTS radical,together with reducing power.Then the correlations between antioxidant activities and contents of two constituents were analyzed by SPSS17.0 software.RESULTS The antioxidant activities of four extracts from N.fordii all showed dose-effect relationships,that of ethyl acetate extract was the strongest,while that of ethanol extract was the weakest.They had some correlations with the content of total flavonoids (0.604 ＜ r ＜ 0.638) and significant correlations with the content of total phenols (r ＞ 0.977).CONCLUSION Ethyl acetate extract from N.fordii can be used for the development of natural antioxidants,and the content of total phenols is the main factor to influence its antioxidant activity.关键词
青天葵/提取物/总黄酮/总酚/抗氧化活性/相关性Key words
Nervilia fordii (Hance) Schltr./extracts/total flavonoids/total phenols/antioxidant activities/correlations分类
医药卫生引用本文复制引用
许海棠,马少妹,张金彦..青天葵4种提取物抗氧化活性与其总黄酮和总酚含量的相关性[J].中成药,2017,39(1):121-125,5.基金项目
国家民委科研项目(14GXZ012) （14GXZ012）
广西民族大学校级项目(2012MDZD042,2014MDQD015) （2012MDZD042,2014MDQD015）
