中成药2018，Vol.40Issue(6)：1233-1237,5.DOI:10.3969/j.issn.1001-1528.2018.06.001
三棱-莪术有效组分配伍液对慢性盆腔炎大鼠盆腔粘连的影响
Effects of active components in compatible solution of Sparganii Rhizoma-Curcumae Rhizoma on pelvic adhesion in rats with chronic pelvic inflammatory disease
摘要
Abstract
AIM To study the influence and action mechanism of effective components in compatible solution of Sparganii Rhizoma-Curcumae Rhizoma on pelvic adhesions of model rats with chronic pelvic inflammatory disease.METHODS Laboratory rats were randomly assigned into six groups,a sham-operated group,a model group,a positive Fukeqianjin tablets group,compatible solution groups (40 g/kg high-dose group,20 g/kg medi um-dose group,and 10 g/kg low-dose group).Except rats of the sham-operated group,those in the other groups were induced into models of chronic pelvic inflammatory disease by mechanical bacterial strain implantation,after which a 20 d corresponding drug administration by oral gavage for each group was initiated.After completion of the last drug administration,Verco criterion was taken as the reference to evaluate pelvic adhesions,HE staining was performed on uterine tissues for pathological scoring,ELASA method was applied to measuring serum IL-1β and TNF-α levels,and Western blot method was used to detect the expression levels of proteins FGF-2 and IGF-1 in uterine tissues.RESULTS Compared with the model group,the high-dose compatible solution group had a much sharper fall in Verco scores (P ＜ 0.05),and remarkably decreased serum IL-1β and TNF-α levels (P ＜ 0.01);both high-dose and medium-dose groups manifested with notably alleviated degenerative necrosis and lowered scores of chronic inflammation infiltration and epithelial hyperproliferation (P ＜ 0.01 or P ＜ 0.05),and a distinct reduction in the expression of proteins FGF-2 and IGF-1 (P ＜ 0.05,P ＜ 0.01).CONCLUSION The significant improvement of the chronic pelvic inflammatory disease in model rats due to the effective components in the compatibile solution of Sparganii Rhizoma-Curcumae Rhizoma may be associated with inhibited release of inflammatory mediators IL-1β and TNF-α,and the reduced expression of proteins FGF-2 and IGF-1 as well.关键词
三棱-莪术有效组分配伍液/盆腔粘连/慢性盆腔炎/FGF-2/IGF-1Key words
effective components of compatible solution of Sparganii Rhizoma-Curcumae Rhizoma/pelvic adhesion/chronic pelvic inflammatory disease/FGF-2/IGF-1分类
医药卫生引用本文复制引用
秦翠梅,于洪建,陈建梅,曹秀莲,任艳青,刘姣..三棱-莪术有效组分配伍液对慢性盆腔炎大鼠盆腔粘连的影响[J].中成药,2018,40(6):1233-1237,5.基金项目
河北省高等学校科学技术研究项目(QN2017112) （QN2017112）
河北省教育厅中药学教学实验示范中心建设项目(2015052265) （2015052265）
