中成药2018，Vol.40Issue(6)：1302-1307,6.DOI:10.3969/j.issn.1001-1528.2018.06.015
一测多评法同时测定玉液消渴颗粒中8种成分
Simultaneous determination of eight constituents in Yuye Xiaoke Granules by QAMS
摘要
Abstract
AIM To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous content determination of 3'-hydroxy puerarin,puerarin,3'-methoxy puerarin,neomangiferin,mangiferin,calycosin-7-O-β-D-glucoside,astragaloside Ⅳ and batatasin Ⅰ in Yuye Xiaoke Granules (Astragali Radix,Dioscoreae Rhizoma,Puerariae lobatae Radix,etc.).METHODS The analysis of methanol extract of this drug was performed on a 30℃ thermostatic Kromasil C1scolumn (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of methanol-acetonitrile (1 ∶ 1) and 0.4％ glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,250,254,274 nm.With astragaloside Ⅳ as an internal standard,the relative correction factors of the other seven constituents were calculated,after which the con tent determination was made.RESULTS Eight constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 97.06％-99.95％ with the RSDs of 0.54％-1.44％.The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This stable and reliable method can be used for the quality control of Yuye Xiaoke Granules.关键词
玉液消渴颗粒/化学成分/一测多评Key words
Yuye Xiaoke Granules/chemical constituents/quantitative analysis of multi-components by single-marker (QAMS)分类
医药卫生引用本文复制引用
陆林杰,姜洪旭,潘科..一测多评法同时测定玉液消渴颗粒中8种成分[J].中成药,2018,40(6):1302-1307,6.基金项目
黔南州科技局项目(黔南科合社字[2012]13) （黔南科合社字[2012]13）
