中成药2018，Vol.40Issue(6)：1307-1312,6.DOI:10.3969/j.issn.1001-1528.2018.06.016
一测多评法同时测定肉豆蔻-8散中5种成分
Simultaneous determination of five constituents in Roudoukou-8 Powder by QAMS
摘要
Abstract
AIM To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous content determination of five constituents in Roudoukou-8 Powder (Myristicae Semen,Auck landiae Radix,Lignum aquilariae Resinatum,etc.).METHODS The analysis of 75％ methanol extract of this drug was performed on a 30 ℃ thermostatic Apollo C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of methanol-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 225,254,273,281 nm.With eugenol as an internal standard,the relative correction factors of the other four constituents were calculated,after which the content determination was made.RESULTS Ellagic acid,eugenol,costunolide,dehydroroma lactone,dehydrodiisoeugenol showed good linear relationships within the ranges of 0.227 0-1.135 2,5.272 2-26.361 0,0.540 8-2.704 0,0.530 4-2.652 0,0.059 0-0.299 5 μg (r ＞0.999 0),whose average recoveries (RSDs) were 96.37％ (2.07％),102.19％ (2.78％),101.66％ (1.66％),103.46％ (1.17％),98.25％ (1.98％),respectively.The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This stable and reliable method can be used for the quality control of Roudoukou-8 Powder.关键词
肉豆蔻-8散/鞣花酸/丁香酚/木香烃内酯/去氢木香内酯/去氢二异丁香酚/一测多评Key words
Roudoukou-8 Powder/ellagic acid/eugenol/costunolide/dehydroroma lactone/dehydrodiisoeugenol/quantitative analysis of multi-components by single-marker (QAMS)分类
医药卫生引用本文复制引用
贾文婷,张媛彦,郭娜,王玉华..一测多评法同时测定肉豆蔻-8散中5种成分[J].中成药,2018,40(6):1307-1312,6.基金项目
国家自然科学基金项目(81360678) （81360678）
内蒙古自治区科技计划项目(1405125) （1405125）
