中成药2018，Vol.40Issue(6)：1320-1325,6.DOI:10.3969/j.issn.1001-1528.2018.06.019
牛膝化学成分及其对细胞增殖影响
Chemical constituents from Achyranthis Bidentata and their effects on cells proliferation
摘要
Abstract
AIM To study chemical constitutes from Achyranthis bidentata Bl.and their effects on cells proliferation.METHODS The aqueous extract from dregs of a decoction of A.bidentata was isolated and purified by Diaion HP-20,Sephadex LH-20,ODS,silica and HPLC colum,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The effects on proliferation of endothelial cells were evaluated by MTT.RESULTS Ten compounds were isolated and identified as cynarasaponin A (1),ginsenoside R0 (2),chikusetsusaponin Ⅳ a (3),chikusetsusaponin Ⅳ a methyl ester (4),chikusetsusaponin Ⅳ a ethylester (5),chikusetsusaponin Ⅳ a butyl ester (6),chikusetsusaponin-1 (7),zingibroside R1 (8),3-O-β-D-glucuronic acid glycosides-oleanolic acid (9),3-O-β-D-glucuronic acid glycosides ethylester-oleanolic acid (10).Compounds 1,6,7,8 exhibited obviously inhibiting effects on HUVEC proliferation.CONCLUSION Compounds 1 and 10 are isolated from A.bidentata for the first time;compounds 1,6,7,8 have strong inhibiting effects on cells proliferation.关键词
牛膝/化学成分/分离鉴定/细胞增殖Key words
Achyranthis bidentata Bl./chemical constituents/isolation and identification/cells proliferation分类
医药卫生引用本文复制引用
张磊,王彦志,赵会丽,胡慧玲..牛膝化学成分及其对细胞增殖影响[J].中成药,2018,40(6):1320-1325,6.基金项目
河南中医药大学科技创新团队、科技创新人才计划资助项目(2015XCXTD02) （2015XCXTD02）
河南中医学院省属高校基本科研业务专项支持计划(2014KYYWF-ZZCXZ-01) （2014KYYWF-ZZCXZ-01）
河南省重大科技专项项目(171100310500) （171100310500）
