中成药2018，Vol.40Issue(6)：1325-1328,4.DOI:10.3969/j.issn.1001-1528.2018.06.020
防风内生真菌DL02次生代谢产物化学成分及其生物活性
Chemical constituents of the secondary metabolites of endophytic fungi DL02 from Saposhnikovia divaricata and their biological activities
摘要
Abstract
AIM To study the chemical constituents of the secondary metabolites of endophytic fungi DL02 from Saposhnikovia divaricata (Turcz.) Schischk.and their biological activities.METHODS The ethyl acetate and methanol extract leavening from S.divaricata was isolated and purified by silica and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antifungal and antibacterial activities were determined by the minimum inhibitory concentration method.RESULTS Seven compounds were isolated and identified as β-sitosterol (1),kojic acid (2),stigmasta-7,22-diene-3β,5α,6α-triol (3),uracil (4),allantoin (5),erythritol (6) and adenosine (7).Compound 4 had strong inhibitory effects on pseudomonas aeruginosa with the MIC values of 31.3 μg/mL.CONCLUSION Compounds 2-5 and 7 are isolated from this fungus for the first time.Compounds 4-5 have strong biological activities.关键词
防风/内生真菌/代谢产物/化学成分/分离鉴定/抗真菌活性/抗细菌活性Key words
Saposhnikovia divaricata (Turcz.) Schischk./endophytic fungi/metabolites/chemical constituents/isolation and identification/antifungal activity/antibacterial activity分类
医药卫生引用本文复制引用
杨秀芳,吴妍,屈子睿,马养民,曹晓晖..防风内生真菌DL02次生代谢产物化学成分及其生物活性[J].中成药,2018,40(6):1325-1328,4.基金项目
陕西省科技统筹创新工程项目(2016KTTSNY03-04) （2016KTTSNY03-04）
