广东海洋大学学报2018，Vol.38Issue(3)：17-24,8.DOI:10.3969/j.issn.1673-9159.2018.03.003
金钱鱼Amhr2基因的克隆及表达分析
Molecular Cloning and Expression Analysis of Anti-Müllerian Hormone Receptor Type Ⅱ (Amhr2) in Spotted Scat (Scatophagus argus)
摘要
Abstract
[Objectives]The spotted scat (Scatophagus argus) Amh (anti-Müllerian hormone)and its specific type Ⅱ receptor Amhr2(anti-Müllerian hormone receptor type Ⅱ) are both involved in gonad development in the vertebrates. However, the gonadal function of Amhr2 is still deficient.[Method]In this study, we have cloned spotted scat Amhr2,and anlyzed its tissue distribution and expression profiles at different ovary development stages by RT-PCR and quantitative Real-time quantitative qPCR,respectively.[Result]The spotted scat Amhr2 full-length ORF is 1533 bp and encodes 510 amino acids. Sequence analysis revealed Amhr2 consists of a signal peptide, atransmembrane domain and a ligand banding domain. Multiple sequence alignment showed that Amhr2 was highly homologous to Amhr2 from Dicentrarchus labrax (73.1％), but was only slightly homologous to human (Homo sapiens) (23.2％) at amino acid level. Phylogenetic analysis revealed that Amhr2 are closest to Dicentrarchus labrax and Larimichthys crocea, which is consistent with the taxology indicating the conversation of Amhr2. In adult spotted scat,Amhr2 is mainly expressed in the gonads, and expressed relatively higher in the testis than the ovary. In female fish,Amhr2 was expressed significantly higher in phase Ⅱ ovary than those of phase Ⅲ and Ⅳ ovaries.[Conclusion]Spotted scat Amhr2 has a higher homology with that of the Perciformes, and may playsan important role in gonads development of the fish.关键词
金钱鱼/Amhr2/基因克隆/基因表达/卵巢发育Key words
Scatophagus argus/Amhr2/gene clone/gene expression/ovary development分类
农业科技引用本文复制引用
王妹,邓思平,陈华谱,朱春华,吴天利,李广丽,江东能..金钱鱼Amhr2基因的克隆及表达分析[J].广东海洋大学学报,2018,38(3):17-24,8.基金项目
湛江市财政资金科技竞争性分配项目(2016A03017) （2016A03017）
广东省自然科学基金(2016A030313743) （2016A030313743）
国家青年基金(31702326) （31702326）
