广东海洋大学学报2018，Vol.38Issue(3)：54-61,8.DOI:10.3969/j.issn.1673-9159.2018.03.009
方格星虫酶解工艺优化及酶解物免疫活性
Optimization of Enzymatic Hydrolysis of Sipunculus nudus L.and Immunoassay of Its Enzymatic Hydrolysis
摘要
Abstract
[Objective]To Optimize the enzymolysis process conditions of Sipunculus nudus L. and explore the effect of enzymatic hydrolysates on cellular immune activity.[Method]Based on the determination of experimental enzymes and single factor experiments, the response surface design was used to establish a mathematical model. With the degree of hydrolysis as the response value, response surface analyses of 3 factors and 3 levels were performed to obtain the optimal conditions for enzymatic hydrolysis. The proliferation of mouse spleen lymphocytes, NO production in peritoneal macrophages of mice and phagocytosis capacity were used to evaluate the effect of the enzymatic hydrolysate on immune cells.[Result]Animal proteases and flavour proteases were used as experimental enzymes. The optimum conditions for the enzymatic hydrolysis ofS. nudusL. were pH 7.0, enzyme addition amount 5000 U/g, ratio of liquid to solid 3:1, and hydrolysis at 55℃ for 5.2 h. The degree of hydrolysis was 38.92％. The enzymatic hydrolysate obtained under these conditions was tested at a concentration of 0.9 mol/mL, the proliferation rate of mouse spleen lymphocytes was 30.57％, and the ability of mouse peritoneal macrophages to generate NO was 56.2μmol/L. The OD540nm value was significantly higher than those in the positive control group (P< 0.01).[Conclusion]The enzymatic hydrolysate ofS. nudus L. has a promoting effect on cellular immunity.关键词
方格星虫/酶解/响应面分析/免疫调节Key words
Sipunculus nudus L./enzymatic hydrolysis/response surface analysis/immuno-regulation分类
轻工纺织引用本文复制引用
孙瑞坤,章超桦,曾少葵,秦小明,曹文红,高加龙,郑惠娜..方格星虫酶解工艺优化及酶解物免疫活性[J].广东海洋大学学报,2018,38(3):54-61,8.基金项目
广东省教育厅创新强校工程项目(2014GKXM047) （2014GKXM047）
广东普通高等学校水产品高值化加工与利用创新团队项目(GDOU2016030503) （GDOU2016030503）
2016年广东省应用型科技研发专项资金项目(2016B020235002) （2016B020235002）
