中国现代中药2017，Vol.19Issue(8)：1076-1082,7.DOI:10.13313/j.issn.1673-4890.2017.8.005
22个白木香倍半萜合酶(AsTPS)基因的健康与伤害诱导表达特性研究
Study on Background and Wound Induced Expression of 22 Sesquiterpene Synthase Genes of Aquilaria sinensis
摘要
Abstract
Objective:TO confirm the expression mode of 22 sesquiterpene synthase in healthy and wound Aquilaria sinensis,and selected the key enzymes for catalyzing agarwood sesquiterpene synthesis.Methods:The branch of A.sinensis were treated by all broken method,the gene expression was tested by PCR and qRT-PCR.Results:1 gene expressed neither in the healthy nor wound trees.3 genes were silenced in healthey trees,but were obviously response to the external stimuli.Other 18 genes expressed in healthy samples among which 7 genes expression increased rapidly and the relative expression level was thousands of times and 11 genes also were induced to express but the relative expression level was dozens to hundreds times in short time after trees were wounded.Conclusion:21 sesquiterpene synthase genes are induced-genes and catalyze synthesis of agarwood sesquiterpenes.Among of them,3 enzymes are the pivotal enzymes for catalyzing sesquiterpene synthesis and 7 enzymes are the key enzymes for sesquiterpene synthesis in early period of agarwood formation in wound A.sinensis.关键词
沉香/白木香/倍半萜合酶/伤害诱导/基因表达Key words
Agarwood/Aquilaria sinensis/sesquiterpene synthase/wound-induce/gene expression引用本文复制引用
吕菲菲,孙佩文,刘培卫,李俊,徐艳红,魏建和..22个白木香倍半萜合酶(AsTPS)基因的健康与伤害诱导表达特性研究[J].中国现代中药,2017,19(8):1076-1082,7.基金项目
国家自然科学基因项目(NO.:81673549),海南省重大科技计划项目(ZDKJ2016004) （NO.:81673549）
海南省自然科学基金(NO.20163150) （NO.20163150）
