中国现代中药2017，Vol.19Issue(8)：1120-1125,6.DOI:10.13313/j.issn.1673-4890.2017.8.014
百药煎传统炮制过程中微生物的分离与初步鉴定及其鞣质水解能力测定
Identification and Isolation of Microorganisms from Chinese Gall Leaven Samples during Its Processing
摘要
Abstract
Objective:To reveal the beneficial microorganisms in processed Chinese gall leaven during their processing by traditional fermentation methods.Methods:Using classical microbial isolation and purification methods,the strains in processed Chinese gall leaven samples were isolated and purified.The preliminary taxonomy of the strains isolated was carried out by investigating the morphological properties,and analyzing and comparing their 16S rDNA or 18S rDNA gene sequences.The ability of degradating tannic acid was determined.Results:Seven species of bacteria,seven species of yeasts and three species of filamentous fungi were isolated and identified in processed Chinese gall leaven samples.The bacteria include Bacillus subtilis,Bacillus cereus,Bacillus lcheniformis,Bacillus,marisflavi,Bacillus megaterium,Bacillus stratosphericus,and Paenibacillus sp.,the yeasts were Hyphopichia burtonii,Kluyveromyces marxianus,Klayveromyces marxianus,Cyberlindnera fabianii,Kodamaea ohmeri,Wickerhamomyces anomalus,and Pichia anomala,and the fungi are Aspergillus niger,Mucor circinelloides,Penicillium spp.By determining the ability of degradating tannic acid,other strains all showed to be positive except for Bacillus subtilis,Bacillus marisflavi,Paenibacillus sp.,Kluyveromyces marxianus HMY3,Cyberlindnera fabianii.Conclusion:Many species of microorganisms exist in processed Chinese gall leaven samples during its processing,in which five species of bacteria,five species of yeasts and three species of filamentous fungi are able to degrade tannic acid,which help to reconstruct modern fermentation technology of processed Chinese gall leaven.关键词
百药煎/炮制/微生物/分离/鉴定Key words
Processed Chinese gall leaven/fermentation/microorganism/isolation/identification引用本文复制引用
胡梦,王瑞生,文雯,刘英,胡海峰..百药煎传统炮制过程中微生物的分离与初步鉴定及其鞣质水解能力测定[J].中国现代中药,2017,19(8):1120-1125,6.基金项目
国家中医药管理局中医药行业专项(201507004) （201507004）
