中国现代中药2017，Vol.19Issue(8)：1131-1135,5.DOI:10.13313/j.issn.1673-4890.2017.8.016
HPLC-DPPH快速测定枳壳抗氧化活性成分
Rapid Determination of Antioxidant Constituents in Aurantii Fructus by HPLC-DPPH Method
摘要
Abstract
Objective:In order to utilize the active ingredients effectively in Aurantii Fructus,a method of determination and evaluation the antioxidant capacity of naringin,hesperidin and neohesperidin simultaneously in Aurantii Fructus was established with high performance liquid chromatography combined with 1,1-diphenyl-2-picrylhydrazyl (HPLC-DPPH) technique.Methods:The determination conditions were as follows:HPLC separation was carried out with an Agilent-ZORBAX SB-C18 column (150 mm × 4.6 mm,5 μm) using 0.04％ phosphoric acid (A)-acetonitrile(B) [0-5 min,12％-20％ B;5-15 min,20％-25％ B;15-25 min,25％-12％ B;5-30 min,12％ B] as mobile phase at a flow rate of 0.8 mL·min-1.The detection wavelength was 283 nm and the column temperature was maintained at 25 ℃.Results:The content of naringin,hesperidin and neohesperidin in Aurantii Fructus was 52.64,2.41,34.27 mg · g-1 separately.The contribution rate of total antioxidant capacity(AA*) was naringin (58.9％) ＞ neohesperidin (38.3％) ＞ hesperidin(2.8％),and the DPPH free radical scavenging rate (AA* *) was naringin(47.8％) ＞ hesperidin (38.1％) ＞ neohesperidin (36.2％).Conclusion:HPLC-DPPH method was efficiently established for the determination and evaluation of the antioxidant compounds of Aurantii Fructus,and this method could provide a new idea for the research and utilization of Aurantii Fructus.关键词
枳壳/柚皮苷/新橙皮苷/橙皮苷/HPLC-DPPH法Key words
Aurantii Fructus/naringin/hesperidin/neohesperidin/HPLC-DPPH assay引用本文复制引用
何英杰,谢红旗,伍睿宇,唐其,程辟,周伊昀..HPLC-DPPH快速测定枳壳抗氧化活性成分[J].中国现代中药,2017,19(8):1131-1135,5.基金项目
国家工信部中药材扶持项目(2015) （2015）
湖南省科技厅重大专项(2015SK1001) （2015SK1001）
湖南省研究生科研创新项目(CX2016B311) （CX2016B311）
湖南省科技项目(2016SK3043) （2016SK3043）
