中国现代中药2017，Vol.19Issue(8)：1140-1145,1150,7.DOI:10.13313/j.issn.1673-4890.2017.8.018
高脂饮食联合链脲佐菌素诱导2型糖尿病合并非酒精性脂肪肝大鼠模型的建立
Establishment of Rat Model of Type 2 Diabetes with Streptozotocin and High-fat Diet
摘要
Abstract
Objective:To establish a rat model of type-2 diabetes with nonalcoholic fatty liver disease (NAFLD) induced by streptozotocin (STZ) and high-fat diet.Methods:35 Wistar male rats were randomly divided into two groups:the control group and the model group,which were given the control diet and the high-fat diet respectively.The rats in the model group were induced diabetes by intraperitoneal injection of 30 mg · kg-1 STZ after 4 weeks.Then we measured and analyzed the changes of fasting blood glucose level (FBG),insulin concentrations,and insulin sensitivity index (ISI) at the 8th week.The rats in the model group that were successfully induced diabetes were further divided into two groups based on FBG:the model group and metformin group.The rats in the metformin group were administered with 200 mg · kg-1 metformin for 4 weeks.At the end of the experiment,the oral glucose tolerance test (OGTF) was performed,also the FBG,ISI,hepatic lipid concentrations were detected.Results:The rats in the model group showed the typical symptoms of diabetes:weight loss,polydipsia and polyuria.Moreover,compared to the control group,the FBG significantly increased (P ＜ 0.001)and ISI significantly decreased (P ＜0.001) in the rats of model group at the 8th and 12th week.At the 12th week,the AUCG significantly decreased (P ＜ 0.001),and the FBG,serum ALT activity significantly decreased (P ＜ 0.01) and hepatic TC concentration obviously decreased (P ＜ 0.5) in the metformin group compared with the model.Conclusion:A stable rat model for type-2 diabetes was successfully established by the induction of high-fat diet combined with the injection of STZ.The effect of metformin in the experiment suggests that the model was proper for evaluating the hypoglycemic agent.关键词
2型糖尿病/非酒精性脂肪肝/高脂饮食/链脲佐菌素Key words
Type-2 diabetes/nonalcoholic fatty liver disease/high-fat diet/streptozotocin引用本文复制引用
孙乐,彭军,杨润梅,许利嘉..高脂饮食联合链脲佐菌素诱导2型糖尿病合并非酒精性脂肪肝大鼠模型的建立[J].中国现代中药,2017,19(8):1140-1145,1150,7.基金项目
协和青年基金资助项目(3332015142) （3332015142）
中国医学科学院医学与健康科技创新工程(2016-I2M-1-012) （2016-I2M-1-012）
