中国现代中药2017，Vol.19Issue(8)：1176-1180,5.DOI:10.13313/j.issn.1673-4890.2017.8.025
正交试验法优选陈皮橙皮苷超声提取工艺
Optimizing Ultrasonic Extraction of Hesperidin from Citri Reticulatae Pericarpium Based on Orthogonal Experiments Design
摘要
Abstract
Objective:To optimize ultrasonic extraction of hesperidin from Citri Reticulatae Pericarpium.Methods:The content of hesperidin was determined by HPLC.The effects of methanol concentration,ultrasonic time,ultrasonic temperature and liquid-material ratio on the content of hesperidin in extracts were investigated by single factor experiments and orthogonal experiments.Results:The decreasing order of importance of the four process conditions affecting hesperidin extraction was methanol concentration,ultrasonic temperature,ultrasonic time,liquid-material ratio.The orthogonal experimental results showed that the optimal conditions were methanol concentration 100％,ultrasonic temperature 70 ℃,ultrasonic time 60 min,liquid-material ratio 80∶1.Conclusion:The optimized process is stable and reasonable,accurate and reliable.This optimum extraction process also had advantages of convenience,high efficiency and cost saving.关键词
陈皮/超声提取/橙皮苷/正交试验Key words
Citri Reticulatae Pericarpium/ultrasonic extraction/hesperidin/orthogonal experiments引用本文复制引用
杨晶,张琳,赵梓邯,侯俊玲,王文全,崔洁,王文端..正交试验法优选陈皮橙皮苷超声提取工艺[J].中国现代中药,2017,19(8):1176-1180,5.基金项目
国家中医药管理局行业专项项目(201107009-03) （201107009-03）
