癌变·畸变·突变2018，Vol.30Issue(3)：204-208,5.DOI:10.3969/j.issn.1004-616x.2018.03.008
红景天提取物对顺铂致肾细胞毒性的保护作用
Protective effect of Rhodiola extract on cisplatin-induced nephrotoxicity
摘要
Abstract
OBJECTIVE:To investigate the protective effect of Rhodiola extract (RE) on cisplatin(DDP)-induced oxidative damage in human embryonic kidney cells (HEK293).METHODS:HEK293 cells were cultured in vitro.The cells were divided into control group,DDP group (0,0.5,1,2,4,8,16,32,64 and 128 mg/L),RE group (0,0.25,0.5,1,2,4,8,16,32 and 64 mg/L),and RE (2-16 mg/L)+DDP (20 mg/L) group.The effect of RE and DDP on the growth of HEK293 cells and the protective effect of RE on DDP-induced cytotoxicity were determined using the CCK-8 assay.Glutathione (GSH) content was measured by dithiobisnitrobenzoate method.Malondialdehyde (MDA) content was measured by thiobarbituric acid method.Superoxide dismutase (SOD) activity was examined by xanthine oxidase method.RESULTS:Compared with the control group,cell survival rates decreased significantly (P＜0.01),and in a dose-dependent manner (r=0.85,P=0.002).The survival rate of RE increased gradually in the range of 0.5-16 mg/L (P＜0.01).When RE concentration was 64 mg/L,the growth of HEK293 cells was inhibited significantly (P＜0.01).The inhibitory effect of 6-16 mg/L RE preconditioning on cell survival with 20 mg/L DDP was significantly higher than that of DDP group (P＜0.01).Compared with DDP group,RE could significantly inhibit the increase of MDA content induced by DDP and the decrease of SOD activity and GSH content (P＜0.01).CONCLUSION:RE treatment enhances the viability of HEK293 cells.In addition,DDP-induced damages in HEK293 cells were remarkedly reversed by RE,and provided protection against DDP-induced nephrotoxicity.关键词
红景天提取物/顺铂/肾毒性/抗氧化Key words
Rhodiola extract/cisplatin/nephrotoxicity/antioxidant分类
医药卫生引用本文复制引用
韩何丹,王海,李艾琳,韩照玉,高丽萍..红景天提取物对顺铂致肾细胞毒性的保护作用[J].癌变·畸变·突变,2018,30(3):204-208,5.基金项目
北京联合大学研究生资助项目 （）
