癌变·畸变·突变2018，Vol.30Issue(3)：214-220,225,8.DOI:10.3969/j.issn.1004-616x.2018.03.010
戊唑醇对秀丽线虫的生殖毒性作用
Reproductive toxicity of tebuconazole on male Caenorhabditis elegans
摘要
Abstract
OBJECTIVE:The aim of this study was to investigate reproductive toxicity of tebuconazoleine tebuconazoleb (TEB) in Caenorhabditis elegans(C.elegans).METHODS:Wild-type(N2) and him-5(e1490) strains from L2-larvae to L4-larvae stages were exposed to TEB at concentrations of 0.1,1.0 and 10.0 μg/L.Brood size and generation time measurements were used to evaluate fertility defects in nematodes.Furthermore,the number of outcross progeny was used to detect reproductive toxicity on male worms.Multiple endpoints were employed to evaluate defects on spermatogenesis,including the number of germ cells in mitosis and meiosis zoon,the number of germs in the germ line and the expression levels of related genes.Sperm size,morphology and activation ability were used to evaluate the adverse effect on spermiogenesis.The level of related genes was detected by real-time fluorescence quantitative PCR.RESULTS:The brood sizes at a concentration of 10.0 μg/L and the number of outcross progeny at concentrations more than 1.0 μg/L were reduced.However,generation times were not changed compared with that from the control group.The number of total germ cells,mitotic cells,meiotic cells and spermatids were less than that from the control group at concentrations of more than 1.0 μg/L.mRNA levels of daf-2,akt-1,and daf-16 were significantly increased at concentrations of 1 and 10 μg/L.Additionally,the mRNA level of age-1 was decreased at the concentrations of 0.1 to 10.0 μg/L compared to the control group.The sperm diameter and cross-sectional sperm area were reduced at concentrations of 0.1-10.0 μg/L,the activation ability of the sperm was declined in the 1.0 μg/L and 10.0 μg/L treatment groups.The expression level of try-5,spe-4,spe-6,fer-1 mRNA were increased significantly and swm-1 mRNA was decreasedcompared with the control group.CONCLUSION:TEB exposure causeds reproductive toxicity in Caenorhabditis elegans by affecting spermatogenesis and spermiogenesis.关键词
秀丽隐杆线虫/戊唑醇/生殖细胞/生殖毒性Key words
Caenorhabditis elegans/tebuconazole/germ cell/reproductive toxicity分类
医药卫生引用本文复制引用
吕荣荣,屈满,岳营,邱月秀,尹立红,李云晖..戊唑醇对秀丽线虫的生殖毒性作用[J].癌变·畸变·突变,2018,30(3):214-220,225,8.基金项目
国家自然科学基金项目(81273123) （81273123）
