癌变·畸变·突变2018，Vol.30Issue(3)：221-225,5.DOI:10.3969/j.issn.1004-616x.2018.03.011
精子与肿瘤细胞融合模型的生物学特性研究
Biological characteristics of a sperm-tumor cell fusion model
摘要
Abstract
OBJECTIVE:To explore the biological characteristics of sperm-tumor cell fusion model.METHODS:A co-cultivation in vitro methods was used to study the time-effectiveness,cell inoculation density dependence and cell type specificity of the fusion model.The chimeric sperm-HeLa monoclonal cells were obtained by using micromanipulation and monoclonal culture method,which was then identified by using PCR test.The chimeric tumor cells in the cervical cancer paraffin were detected by amplification Y chromosome specific gene TSPY.RESULTS:Fusion between the sperm and tumor cells generally occurred in 1.5 hours after co-cultivation,the fusion rates was inversely proportional to the cell inoculation density,and the tumor cells who derived from an adenocarcinoma had a higher rate of cell fusion than other tumor cells and normal controls.One chimeric tumor tissue was observed in 20 cases of cervical cancer specimens,and one righteous mutation was found in TSPY gene.CONCLUSION:Cell fusion between the sperm and tumor cells displayed time-effectiveness,cell inoculation density dependence and cell type specificity.The chimeric tumor cells could be found in cervical cancer specimens.关键词
精子/肿瘤细胞/细胞融合/嵌合细胞Key words
sperm/tumor cells/cell fusion/chimeric cells分类
生物科学引用本文复制引用
陈强,靳广毅,林桂淼,刘好,许改霞,王晓梅..精子与肿瘤细胞融合模型的生物学特性研究[J].癌变·畸变·突变,2018,30(3):221-225,5.基金项目
国家科技部国家重点研发计划项目(2016YFC0904601) （2016YFC0904601）
深圳市基础研究项目(JCYJ20170303160906960) （JCYJ20170303160906960）
深圳市国际合作项目(GJHZ20170313111237888) （GJHZ20170313111237888）
深圳市科创委布局项目(基20160084) （基20160084）
