草业学报2018，Vol.27Issue(5)：141-152,12.DOI:10.11686/cyxb2017433
盐旱交互对燕麦种子萌发及幼苗生理特性的影响
Effects of interaction between Ca2+ salt and drought stress on seed germination and seedling physiology of oats
摘要
Abstract
The research aimed to study the adaptation mechanisms of oats (Avena sativa) to exposure to soil environments with high levels of calcium salts and drought,as in karst areas.PEG-6000 was used to set up solutions with 0 (CK),-0.2,-0.4,-0.6,-0.8,-1.2 MPa osmotic potential,to simulate a range of drought environments in karst areas.Similarly,calcium chloride (CaCl2) solutions of 0 (CK),50,100,150,200 mmol · L-1 were prepared to provide exogenous calcium and simulate the range of soil calcium environments in karst areas.The effects of low osmotic potential and Ca2+ exposure singly and their interaction effect on the seed germination characteristics and seedling physiological characteristics of oats were studied.Both low and medium levels of osmotic stress and exposure to elevated Ca2+,singly or in combination,promoted the germination of oat seeds and enhanced the activities of superoxide dismutase,catalase,peroxidase and glutathione peroxidase in seedlings.This was especially true in seedlings subjected to 100 mmol · L 1 CaCl2 and-0.6 MPa PEG interactive stress,where the germination rate increased significantly by 42.17％ compared with the CK treatment.Two-way ANOVA showed that the effect of salt-drought interaction stress on oats was more significant than that of either single stress (P＜0.05).Through comprehensive data analysis,oat tolerance of the stressors tested was found to be as follows:interaction stress＞single PEG stress＞single CaCl2 stress.The exposure levels tolerated by oats under CaCl2,PEG and interaction stresses,were 100 mmol · L-1,-0.6 MPa,100 mmol · L-1 CaCl2 +-0.6 MPa PEG,respectively.The above results show that in karst mountain areas,medium-low concentrations of Ca2+ and ormoderate drought typical in these areas can promote seed germination and seedling growth of oats.This study provides reference information for the introduction and cultivation of oats in karst areas and for further research into breeding for stress resistance.关键词
燕麦/干旱胁迫/外源钙/种子萌发/幼苗生理Key words
oat/drought stress/exogenous calcium/seed germination/seedling physiology引用本文复制引用
张宇君,赵丽丽,王普昶,陈超..盐旱交互对燕麦种子萌发及幼苗生理特性的影响[J].草业学报,2018,27(5):141-152,12.基金项目
国家科技支撑计划(2014BAD23B03),国家自然科学基金(31560664),贵州省农业攻关计划项目(黔科合NY字[2014]3048号、黔科合支撑[2016]2516号)和贵州大学研究生创新基金(研农2017002号)资助. （2014BAD23B03）
