重庆医学2018，Vol.47Issue(17)：2257-2260,2265,5.DOI:10.3969/j.issn.1671-8348.2018.17.002
miRNA-24-3P靶向调节SP1对RAW264.7细胞功能的影响
miRNA-24-3P affect RAW264.7 cell function by modulating SP1
摘要
Abstract
Objective To discuss the mechanism of miR-24-3P in the process of acute respiratory distress syndrome (ARDS).Methods miR-24-3P mimics,miR-24-3P inhibitor and their negative controls were transfected in RAW264.7 cells respectively:The relative expression of miR-24-3P in each cell was detected.The relative expression of TNF-α,IL-6,SP1 and NF-κB were detected in each cell,and the regulation of miR-24-3P on the target gene SP1 was detected by dual luciferase reporter gene.Results The expression level of TNF-α mRNA and IL-6 mRNA in each group was statistically significant (P＜0.05).The relative expression level of SP1 mRNA and NF-κB mRNA in each group was not statistically significant (P＞0.05).The expression of SP1 in each group was statistically significant (P＜0.05).The gene analysis of dual luciferase reporter showed that the fluorescence activity was significantly inhibited after cotransfection of miR-24-3P and SP1 in HEK293 cells(P＜0.05).Conclusion miR-24-3P can play an important role in the process of ARDS by influencing the translation of SP1 gene and affecting the release of inflammatory mediators.关键词
miR-24-3P/急性呼吸窘迫综合征,成人/SP1Key words
miRNA-24-3P/respiratory distress syndrome, adult/SP1分类
医药卫生引用本文复制引用
程东良,梁媛,陈衍晨,卿娣..miRNA-24-3P靶向调节SP1对RAW264.7细胞功能的影响[J].重庆医学,2018,47(17):2257-2260,2265,5.基金项目
河南省医学科技攻关计划项目(201702153). （201702153）
