重庆医学2018，Vol.47Issue(17)：2270-2274,5.DOI:10.3969/j.issn.1671-8348.2018.17.005
大鼠热应激对细胞免疫学的影响
The effection of heat stress on cell immunology in rats
周文芸 1吕芷娴 1曾黎峰 1何丹 1胡国柱1
作者信息
- 1. 江西省人民医院临床医学研究所,南昌330006
- 折叠
摘要
Abstract
Objective To investigate the effect of heat stress on the expression of Toll-like receptor 4 (TLR4) and peripheral blood T regulatory cells (Treg) in splenic cells of rats.Methods Senventy-two SD rats were divided into 20 ℃ control group and 37 ℃ group.Each group was divided into non stimulation,bacterial lipopolysaccharide (LPS) stimulation and concanavin A (Con-A) stimulation subgroup.Each subgroup had 1,12,48 h and 168 h observation points,and flow cytometry was used to determine the level of TLR4 and Treg.Results The TLR4+ immunocompetent cells in the spleen was decreased from 1 h to 168 h in every subgroup of the 37 ℃ group compared to the 20 ℃ control group (P＜0.05).The level of CD4+CD25+ Treg in the peripheral blood in rats from 1 to 48 h was significantly decreased (P＜0.05) and slightly increased at 168 h in LPS stimulation subgroup in the 37 ℃ group compared to the 20 ℃ control group.The level of CD4+ CD25+ Foxp3+ Treg in the peripheral blood in rats at 12 h were significantly increased in non-stimulation and concanavalin A (Con-A) stimulation subgroups in the 37 ℃group,and were significantly decreased at 168 h in every subgroup of the 37 ℃ group compared with 20 ℃ control group (P＜0.05).The level of CD8+CD25+ Treg in the peripheral blood in rats was significantly increased at 1 h and 168 h in the 37 ℃ hot and humid group in the every subgroup whencompared with the 20 ℃ control group (P＜0.05).The level of CD8+ CD25+Foxp3+ Treg in the peripheral blood in rats was significantly decreased at 1 h and 48 h in the every subgroup,and was significantly increased at 12 h and 168 h in the non-stimulation and LPS stimulation subgroups in the 37 ℃ group compared to the 20 ℃ control group (P＜0.05).Conclusion High temperature and damp heat can destroy the innate immunity and alter the functional state of adaptive immunity in rats.关键词
SD大鼠/脾脏/免疫细胞/TLR4/Treg/热应激/LPS/Con-AKey words
rats, sprague-dawley/spleen/immunocompetent cells/Toll-Like receptor 4/Treg/heat stress/lipopolysaccharides/Con-A分类
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周文芸,吕芷娴,曾黎峰,何丹,胡国柱..大鼠热应激对细胞免疫学的影响[J].重庆医学,2018,47(17):2270-2274,5.
