重庆医学2018，Vol.47Issue(17)：2275-2278,2284,5.DOI:10.3969/j.issn.1671-8348.2018.17.006
ica操纵子对表皮葡萄球菌在不同骨科植入物表面黏附和生物膜形成的影响
Effect of ica operator on adherence and biofilm formation of Staphylococcus epidermidis at orthopedic implants
摘要
Abstract
Objective To investigate the effect of ica operon on adherence and biofilm formation of Staphylococcus epidermidis(S.epidermidis)at orthopedic implants,and to provide theoretical basis for the use of clinical orthopedic materials.Methods The icaADBC genes of S.epidermidis were amplified by PCR.The expression of ica genes was detected by fluorescence quantitative PCR.The contents of polysaccharide intercellular adhesion (PIA) were determined by phenol-sulfuric acid method.The ability of bacterial adherence was detected by colony counting method and the ability of biofilm formation was measured by crystal violet staining method.Results The icaADBC genes were presented and expressed in eleven S.epidermidis strains.The strains with high icaABDC gene expression had higher PIA product.S.epidermidis isolates had the strongest adherence and biofilm formation ability on bone material,followed by titanium and stainless steel.The expression of icaA gene correlated with the biofilm formation of bacteria on three biomaterials.Conclusion S.epidermidis isolates had the different adherence and biofilm formation on different biomaterial implants.Ica gene regulates the biofilm formation of S.epidermidis by regulating the expression of PIA.关键词
表皮葡萄球菌/ica操纵子基因/植入材料/细胞黏附/生物膜Key words
staphylococcus epidermidis/ica gene/prostheses and implants/cell adhesion/biofilms分类
医药卫生引用本文复制引用
陈鹏,李波,彭智,高淦,陈盛..ica操纵子对表皮葡萄球菌在不同骨科植入物表面黏附和生物膜形成的影响[J].重庆医学,2018,47(17):2275-2278,2284,5.基金项目
贵州省科技合作计划项目(黔科合LH字7123号). （黔科合LH字7123号）
