重庆医学2018，Vol.47Issue(17)：2285-2289,5.DOI:10.3969/j.issn.1671-8348.2018.17.008
microRNA-622调控DYRK2在结肠癌中表达并促进结肠癌细胞SW1116侵袭转移
MicroRNA-622 regulates DYRK2 expression in colon cancer and promotes migration in colon cancer cell SW1116
摘要
Abstract
Objective To investigate the expressiorn of microRNA-622(miR-622) and dual specificity tyrosine phosphorylation-regulated kinase 2 (DYRK2) in colon cancer tissues and cell lines and explore the effect of miR-622 on SW11l6 cells migration and invasion.Methods Eighty-two colon cancer tissues and paired para-tumor tissue specimens were collected.C.olon cancer cell line SW1116,SW480 and normal human colon epithelial cell line NCM460 were cultured.MiR-622 was detected by using Real time PCR,DYRK2 expression was measured by using immunohistochemistry,Real time PCR anid Western blot in tissue level and cell level,respectively.The relation of miR-622 and DYRK2 was analyzed by Pearson correlation analysis.miR-622 mimics transfection was conducted to up-regulate miR-622,while negative control,NC group were transfected with control sequence.Expression of DYRK2 was evaluated by using Real time PCR and Western blot,while Transwell chamber assays were used to assess the migration ability changes.Results Real time PCR and Western blot results showed that miR-622 mRNA was highly expressed in colorectal cancer tissue and colon cancer cell SW1116,whereas DYRK2 mRNA and protein were lowly expressed when compared with paracancerous tissue and normal colonic epithelial cell line NCM460.An obvious negative correlation was showed between miR-622 and DYRK2(r=0.916,P＜0.01).Compared to NC group,DYRK2 mRNA and protein expression were down-regulated after transfection of miR-622 mimics,which was observerd through Real time PCR and Western blot(P＜0.01).Correspondingly,compared to NC group,the migration ability of SW116 was remarkably enhanced after transfection of miR-622 mimics(P＜0.01).Conclusion The expression of miR-622 is high and DYRK2 is low in colon cancer.Up-regulation of miR-622 could negatively regulate DYRK2 expression and promote SW1116 cells migration.关键词
微RNA-622/DYRK2/结肠肿瘤/肿瘤浸润/肿瘤转移Key words
microRNA-622/DYRK2/colonic neoplasms/neoplasm invasiveness/neoplasm metastasis分类
医药卫生引用本文复制引用
魏晰麟,杜健峰,王勇,卢佳宁,娄琳,孙洁,周忠笑,张健,曾宪东..microRNA-622调控DYRK2在结肠癌中表达并促进结肠癌细胞SW1116侵袭转移[J].重庆医学,2018,47(17):2285-2289,5.基金项目
辽宁省社会发展公关计划项目(2015020377) （2015020377）
沈阳市科技创新专项基金(F15-139-9-07) （F15-139-9-07）
沈阳医学院科技基金项目(2018011). （2018011）
