解剖学杂志2018，Vol.41Issue(3)：249-252,4.DOI:10.3969/j.issn.1001-1633.2018.03.001
透射电镜在糖尿病大鼠肠上皮细胞连接分析中的应用
Application of transmission electron microscopy in the analysis of intestinal epithelial cell connections in diabetic rats
摘要
Abstract
Objective:To investigate the application of transmission electron microscopy (TEM) in the analysis of intestinal epithelial cell connectivity in diabetic rats.Methods:A diabetic rat model was established by high fat diet combined with intraperitoneal injection of STZ.The blood glucose in tail blood of rats was detected by Roche glucose meter.The pathological changes of the ileum epithelial cell connections were observed by transmission electron microscopy.The expression levels of claudin,occludin and ZO-1 in the ileum of rats were detected by Western blotting.Results:The model of diabetic rats was successfully constructed.Compared with the normal control group,the blood glucose value was significantly higher in diabetic rat models,and the blood sugar remained on a high level after 4 weeks of continuous feeding.Compared with the normal control group,diabetic rats showed a little relaxation in intestinal epithelial tight junctions between cells,and decreased electron density.The gap of intermediate junction and gap junction appeared abnormally wide.The expression level of cell junction associated protein claudin,occludin,ZO-1 were significantly lower.Conclusion:Transmission electron microscopy (TEM) can be used for pathological analysis of intestinal epithelial cell junction in diabetic rats,and is of great significance for evaluation of intestinal mucosal barrier function in diabetic rats.关键词
糖尿病/细胞连接/细胞连接相关蛋白/透射电镜/大鼠Key words
diabetes mellitus/intestinal epithelial cell connection/cell junction associated protein/transmission electron microscopy/rat引用本文复制引用
常志尚,毋亚仙,刘立娜,谭金山,刘颖,梁惠..透射电镜在糖尿病大鼠肠上皮细胞连接分析中的应用[J].解剖学杂志,2018,41(3):249-252,4.基金项目
国家自然科学基金(81573137) （81573137）
青岛大学通识教育核心课程建设项目 （）
青岛大学创新型教学实验室研究项目 （）
