解剖学杂志2018，Vol.41Issue(3)：276-279,4.DOI:10.3969/j.issn.1001-1633.2018.03.008
原代大鼠脑微血管内皮细胞的培养及鉴定
Primary culture and identification of microvascular endothelial cells of rat brains
摘要
Abstract
Objective:To establish a method that can successfully separate and cultivate microvascular endothelial cells of rat brains in vitro.Methods:The microvascular segments of the rat brain were obtained from 7 to 10 d SD rats by homogenation,bovine serum albumin gradient centrifugation and digestion with collagenase type Ⅱ and then they were cultured in carbon dioxide incubator.The cultured cells were identified by cell morphology observation and immunohistochemical detection of Ⅷ factor-related antigen.Results:Under the inverted microscope,the cells in vitro cultured for 24 h to 48 h,emerged from the microvascular section of the wall.The cells were fusiform-shaped or polygonal-shaped,and proliferated in a clustered monolayer.The typical "paving pebbles" sample was observed after the cells became dense.The correlation antigen of Ⅷ factor was positive,the cytoplasm was brown,and the positive cells accounted for more than 99％.Conclusion:The method can successfully separate and cultivate microvascular endothelial cells of rat brains in vitro.关键词
脑/微血管内皮细胞/原代培养/Ⅷ因子相关抗原/大鼠Key words
brain/microvascular endothelial cells/primiary culture/Ⅷ factor related antigen/rat引用本文复制引用
马华根,唐元瑜,纪立金..原代大鼠脑微血管内皮细胞的培养及鉴定[J].解剖学杂志,2018,41(3):276-279,4.基金项目
国家自然科学基金(81373528,81072714) （81373528,81072714）
福建省自然科学基金(2017J01545) （2017J01545）
2018年福建省大学生创新创业训练计划项目(201810393033) （201810393033）
