解剖学杂志2018，Vol.41Issue(3)：303-306,4.DOI:10.3969/j.issn.1001-1633.2018.03.014
丹参酮ⅡA对帕金森病大鼠多巴胺能神经元的作用
Effects of Tanshinone ⅡA on dopaminergic neurons in rats with Parkinson's disease
摘要
Abstract
Objective:To observe the effects of Tanshinone ⅡA on the expression of tyrosine hydroxylase (TH),glucoseregulated protein 78 (GRP78),caspase-3 and apoptosis of rats with Parkinson's disease (PD),and to explore the role and mechanism of Tanshinone ⅡA on rats with Parkinson's disease.Methods:Adult SD rats were randomly divided into control group,sham group and PD model group.PD model group was randomly and evenly divided into saline group (NS group) and treatment group (TSA group).The PD model group was established by subcutaneous injection of rotenone in the neck and back area,while the injection of sunflower oil emulsion without rotenone at the same point and with the same quantity as the PD model group was applied in the sham group.The control group was not given any intervention.Nature saline (1 ml) and tanshinone ⅡA (20 mg/kg) were given through intraperitoneal injection respectively for 14 days in the PD model group.TH,GRP78 and caspase-3 were measured with immunohistochemical staining,and apoptosis in the substantia nigra was tested by TUNEL method.Results:Compared with the control group and sham group,there was typical PD ethologic change in PD model group.Compared with the control group,TH positive neuron decreased,while the expression of GRP78,caspase-3 and the number of apoptotic cells increased significantly in the substantia nigra in the saline group.Compared with the saline group,TH positive neurons increased,while the expression of GRP78,caspase-3 and the number of apoptotic cells decreased significantly in the substantia nigra in the TSA group.Conclusions:Tanshinone ⅡA can mitigate the loss of dopaminergic neurons in the PD model rats induced by rotenone,which may be related with reducing the stress response of endoplasmic reticulum.关键词
帕金森病/鱼藤酮/丹参酮ⅡA/内质网应激/大鼠Key words
Parkinson's disease/rotenone/tanshinone ⅡA/endoplasmic reticulum stress/rat引用本文复制引用
田新红,徐玉英,郝莉..丹参酮ⅡA对帕金森病大鼠多巴胺能神经元的作用[J].解剖学杂志,2018,41(3):303-306,4.基金项目
河南省高等学校重点科研项目(18A310016) （18A310016）
河南中医学院省属科研业务费专项青年项目(2014KYYWF-QN14) （2014KYYWF-QN14）
