临床肝胆病杂志2018，Vol.34Issue(6)：1248-1252,5.DOI:10.3969/j.issn.1001-5256.2018.06.023
1型自身免疫性肝炎患者外周血T淋巴细胞PD-L1的表达情况及其与肝脏炎症活动度的相关性分析
Expression of programmed death -ligand 1 on peripheral blood T cells and its correlation with liver inflammationgrade in patients with type 1 autoimmune hepatitis
摘要
Abstract
Objective To investigate the expression of programmed death -ligand 1(PD -L1) on the surface of peripheral blood CD4 + and CD8 + T cells and its correlation with liver inflammation grade in patients with type 1 autoimmune hepatitis (AIH).Methods A total of 62 patients with type 1 AIH (43 in the active stage and 19 in the remission stage) who were admitted to Mengchao Hepatobiliary Hospital of Fu - jian Medical University from January 2014 to August 2017 were enrolled, and 20 healthy persons were enrolled as control group .Flow cytometry was used to measure the expression of PD -L1 on the surface of eripheral blood CD4 + and CD8 + T cells.The t -test or the Mann - Whitney U test was used for the comparison of continuous data between two groups , and a one -way analysis of variance and the least signifi - cant difference t -test or the Kruskal -Wallis H test were used for the comparison between multiple groups ; the chi -square test was used for comparison of categorical data between groups ; Pearson correlation and Spearman rank analysis was performed to investigate correlation .Results Compared with the control group, the type 1 AIH group had significantly higher expression rates of PD -L1 on the surface of pe- ripheral blood CD4 + and CD8 + T cells (10.94% ±4.52%/9.93% ±3.32% vs 5.42% ±2.64%/4.86% ±2.16%, t =7.12 and 6.41,both P <0.05); compared with the remission stage group and the control group , the active stage group had significantly higher expression rates of PD -L1 on the surface of peripheral blood CD4 + and CD8 + T cells (12.45% ±5.61%/11.13% ±4.63% vs 7.88% ±3.85%/ 7.36% ±3.12% and 5.42% ±2.64%/4.86% ±2.16%, F =15.76 and 13.54, both P <0.05), and the remission stage group also had significantly higher expression rates than the control group (both P <0.05).There were gradual increases in the expression rates of PD -L1 on the surface of peripheral blood CD4 + and CD8 + T cells in the patients with type 1 AIH in G0 group (7.88% ±3.85% and 5.36% ± 3.12%), G1 -2 group (10.13% ±4.74% and 10.45% ±4.34%), and G3 -4 group (15.63% ±5.98% and 14.73% ±5.12%) (χ2 = 12.14 and 10.32, P <0.05).In the patients in the active stage of type I AIH, the expression rates of PD -L1 on the surface of peripheral blood CD4 + and CD8 + T cells were positively correlated with total bilirubin , alanine aminotransferase, aspartate aminotransferase, gamma -glutamyl transpeptidase, and immunoglobulin G (CD4 + T cells: r =0.687, 0.736, 0.702, 0.647, and 0.824, all P <0.01; CD8 + T cells: r =0.673, 0.719, 0.680, 0.631, and 0.817, all P <0.01); the expression rates of PD -L1 on the surface of peripheral blood CD4 + and CD8 + T cells were also positively correlated with liver inflammation grade (rs =0.768 and 0.721, both P <0.05).Conclusion In patients with type I AIH, high expression of PD -L1 on peripheral blood T cells is closely associated with liver inflammation grade , suggesting that PD -L1 may be involved in the development and progression of type 1 AIH.关键词
自身免疫性肝炎/T淋巴细胞/程序性细胞死亡因子配体1Key words
hepatitis/autoimmune/T-lymphocytes/programmed death-ligand 1分类
医药卫生引用本文复制引用
熊克宫,柯坤宇,孔金峰,柳丽娟,陈丽芳,林小钦..1型自身免疫性肝炎患者外周血T淋巴细胞PD-L1的表达情况及其与肝脏炎症活动度的相关性分析[J].临床肝胆病杂志,2018,34(6):1248-1252,5.基金项目
福州市卫生计生科技计划项目(2016-S-wq7) （2016-S-wq7）
福州市科技强院建设项目(2017-S-131-1) （2017-S-131-1）
福建省科技计划项目(2018D0006) （2018D0006）
