临床肝胆病杂志2018，Vol.34Issue(6)：1273-1276,4.DOI:10.3969/j.issn.1001-5256.2018.06.028
非酒精性脂肪性肝炎大鼠模型中小檗碱抗氧化效应的观察
Antioxidant effect of berberine in a rat model of nonalcoholic steatohepatitis
蒋煜 1胡居龙 1马佳丽 1李坪 1周玉玲 1梁秀霞 1艾正琳 1姚树坤2
作者信息
- 1. 首都医科大学附属北京地坛医院消化内科,北京100015
- 2. 卫生部中日友好医院,北京100029
- 折叠
摘要
Abstract
Objective To investigate the antioxidant effect of berberine in a rat model of nonalcoholic steatohepatitis (NASH) and an in vitro model of oxidative stress induced by H2O2.Methods High -fat diet was used to establish a rat model of NASH .After three weeks of treatment with berberine 18 mg/kg/d by gavage, HE staining was used to observe liver pathological changes , and the activities of glutathione (GSH) and superoxide dismutase (SOD) and the level of malondialdehyde (MDA) in liver tissue were measured.An in vitro model of oxidative stress induced by reactive oxygen species (ROS) due to H2 O2 was established; flow cytometry was used to measure the level of ROS ,and the activities of SOD, GSH, lactate dehydrogenase (LDH), and chloramphenicol acetyltransferase (CAT) in hepatocytes were measured.A one -way analysis of variance was used for comparison of continuous data between groups , and the Bonferroni test was used for fur - ther comparison between two groups; the t -test was used for comparison of continuous data between two groups .Results NASH rats had significant improvements in the extent of infiltration of inflammatory cells , focal necrosis, and ballooning degeneration of hepatocytes after berberine treatment.Compared with the NASH model group, the berberine group had significant increases in the activities of GSH (29.8 ± 2.4 U/mg vs 19.9 ±1.3 U/mg, P <0.001) and SOD (26.6 ±1.9 μg/mg vs 19.7 ±1.4 μg/mg, P <0.001) and a significant reduction in the level of MDA (19.8 ±1.5 nmol/mg vs 24.0 ±2.0 nmol/mg, P <0.001).In the in vitro model of oxidative stress induced by H2O2 ,the H2O2 group had a significant increase in the level of ROS compared with the berberine +H2O2 group (69.8% ±1.9% vs 50.4% ± 6.5%, t =24.42, P =0.008).Compared with the H2 O2 group, the berberine +H2O2 group had significant increases in the activities of SOD (25.5 ±1.3 μg/mg vs 18.8 ±1.0 μg/mg, P <0.001) and GSH (27.1 ±0.6 U/mg vs 16.79 ±3.8 U/mg, P <0.001) in hepato- cytes; there was no significant difference in the level of LDH between the berberine +H2O2 group and the H2O2 group (P =0.103), and there was no significant difference in the activity of CAT across all groups (F =3.76, P =0.060).Conclusion Both in vivo and in vitro models show that berberine has a good antioxidant effect , possibly by increasing the activities of antioxidant enzymes .关键词
非酒精性脂肪性肝炎/小檗碱/氧化性应激/大鼠,wistarKey words
nonalcoholic steatohepatitis/berberine/oxidative stress/rats/wistar分类
医药卫生引用本文复制引用
蒋煜,胡居龙,马佳丽,李坪,周玉玲,梁秀霞,艾正琳,姚树坤..非酒精性脂肪性肝炎大鼠模型中小檗碱抗氧化效应的观察[J].临床肝胆病杂志,2018,34(6):1273-1276,4.
