中国海洋大学学报（自然科学版）2018，Vol.48Issue(6)：45-54,10.DOI:10.16441/j.cnki.hdxb.20170186
长牡蛎Fem-1基因cDNA克隆和表达分析
Cloning and Expression Analysis of Fem-1 Gene of Pacific Oyster (Crassostrea gigas)
摘要
Abstract
We isolated the complete eDNA sequences of Fem-1b and Fem-1c gene of Pacific oyster (Crassostrea gigas) using RACE technique,and analyzed their deduced protein sequences and the phylogenetic relationship of these proteins.In addition,using RT-PCR technique,we did expression analysis in the gonads collected monthly from May 2015 through April 2016,different tissues collected in the summer of 2015 and embryonic larvae at different developmental stages.Results showed that the full length cDNA of Cg-Fem-1b was 2 580 bp encoding 636 amino acids while that of Cg-Fem-1c was 2,417 bp encoding 622 amino acids.The protein structure prediction showed that two genes contained 6 and 7 ankytin repeat motifs,respectively.RT-PCR results showed that the expression of two genes in testis was significantly higher than their expression in other tissues (P＜0.05).The expression of Cg-Fem-1b in testis was significantly higher than its expression in other groups in June and July while the expression of Cg-Fem-1c in testis was significantly higher than its expression in other groups in March and April (P＜0.05).Two genes highly expressed at the early stages of embryo development in comparison with their expression at middle and late stages,which reached the highest at blastocyst stage (P＜0.05).We inferred that Fem-1b and Fem-1c genes may be involved in sex determination and differentiation.关键词
长牡蛎/Fem-1b/Fem-1c/基因克隆/表达分析Key words
Crassostrea gigas/Fem-1b/Fem-1c/cDNA isolation/expression analysis分类
农业科技引用本文复制引用
周祖阳,李琪,于红,孔令锋..长牡蛎Fem-1基因cDNA克隆和表达分析[J].中国海洋大学学报（自然科学版）,2018,48(6):45-54,10.基金项目
国家自然科学基金项目(31772843) （31772843）
泰山学者种业计划专家项目 （）
山东省科技发展计划项目(2016ZDJS06A06)资助 Supported by National Natural Science Foundation of China (31772843),Taishan Scholars Seed Project of Shandong,Shandong Province (2016ZDJS06A06) （2016ZDJS06A06）
