中国海洋大学学报（自然科学版）2018，Vol.48Issue(7)：10-19,10.DOI:10.16441/j.cnki.hdxb.20170238
弗尼斯弧菌PCR检测方法的建立及应用
Establishment and Application of a PCR Based Method for Detection of Vibrio furnissii
摘要
Abstract
Vibrio furnissii is recognized as a new foodborne pathogenic bacterium by scholars both in China and abroad in recent years.It poses a potential threat to the heath of global public and food safety.Conventional PCR and real-time fluorescence PCR techniques were applied to the rapid detection of V.furnissii by designing primers and probe targeting the toxS gene.The specificity and sensitivity of the methods were investigated.The results showed that the detection specificity with conventional PCR and real-time fluorescence PCR was high;the detection limit of the conventional PCR and real-time fluorescence PCR was 2.4 × 103 CFU/mL and 24 CFU/mL,respectively.The detection sensitivity of the two methods was 10 times and 1 000 times of the traditional culture method.In addition,the technique enabledthe accurate recognition of V.furnissii from food products (6 positive products/384 food products and aquatic products).The specificity (100％) sensitivity (24 CFU/mL-2.4× 103 CFU/mL) fully satisfied the requirements for the detection of V.furnissii.Two PCR method targeting V.furnissii toxS gene was established in this study,which can be applied to detecting V.furnissii in food products.关键词
弗尼斯弧菌/普通PCR/实时荧光PCR/toxS基因/快速检测Key words
Vibrio furnissii/conventional PCR/real-time fluorescence PCR/toxS gene/rapid detection分类
农业科技引用本文复制引用
王殿夫,何平,王青,于洋,卢福荣,冯华炜,麻丽丹..弗尼斯弧菌PCR检测方法的建立及应用[J].中国海洋大学学报（自然科学版）,2018,48(7):10-19,10.基金项目
国家质检总局科研项目(2012IK170)资助 Supported by Quarantine of the People's Republic of China Fund (2012IK170) （2012IK170）
