中国海洋大学学报（自然科学版）2018，Vol.48Issue(7)：20-28,9.DOI:10.16441/j.cnki.hdxb.20170296
溶解氧水平对花鲈幼鱼氧化应激与能量利用的影响及生理机制
Effects of Dissolved Oxygen Levels on Oxidative Stress Response and Energy Utilization of Juvenile Chinese Sea Bass (Lateolabrax maculatus) and Associate Physiological Mechanisms
摘要
Abstract
In order to study the effect of hypoxia on the hematological physiology,oxidative stress response and energy utilization of Chinese sea bass (Lateolabrax maculatus),the fish were treated at dissolved oxygen content of ((1.52 ± 0.24) mg/L) for 3 h,6 h,12 h and 24 h and then recovered at the normal ((7.72±0.18) mg/L) for 3 h and 12 h with the blood cells,serum metabolites and the levels of antioxidant enzymes and energy supply substances in different tissues determined,respectively.Hypoxia increased the blood cell count (WBC),red blood cell count (RBC),platelet count (PLT) and hemoglobin content (HGB) significantly (P＜0.05) and decreased to the normal level after recovery at normal dissolved oxygen content.Glutamate pyruvic transaminase(ALT),aspartate transaminase (AST),lactate dehydrogenase(LDH) and triglyceride (TG) were consistent with the change of blood cells.Alkaline phosphatase (ALP),total protein (TP),albumin (ALB) and total cholesterol (TC) significantly decreased (P＜0.05)and then returned to the normal.The activity of superoxide dismutase (SOD) and glutathione-S-transferase (GST) in liver increased first and then decreased.The change of GST in liver tissue is more sensitive to hypoxia,which can be used as an enzyme indicator of L.maculatus suffering the oxidative stress.The contents of glycogen and lactic acid in liver significantly decreased after hypoxia treatment (P＜0.05).The activities of SOD,GST,catalase (CAT) and malondialdehyde (MDA) in gill increased differentially.The activity of SOD and CAT in muscle significantly decreased (P＜0.05) while the contents of MDA and glycogen in muscle significantly increased (P＜0.05).The results of this study showed that hypoxia stress can cause significant oxidative injury to juvenile L.maculatus and change the blood biochemical indexes,the activities of related enzymes in different tissues and energy supply.After being recovered to normal dissolved oxygen,the changed parameters can gradually return to the normal through the physiological regulation of fish.关键词
花鲈/低氧胁迫/血液生理生化/氧化应激/能量利用Key words
Lateolabrax maculatus/hypoxia stress/hematological physiology/oxidative stress/energy utilization分类
农业科技引用本文复制引用
常志成,王晓龙,温海深,张美昭,李吉方,李昀,张凯强,王伟,刘阳,田源..溶解氧水平对花鲈幼鱼氧化应激与能量利用的影响及生理机制[J].中国海洋大学学报（自然科学版）,2018,48(7):20-28,9.基金项目
现代农业产业技术体系专项(CARS-47) （CARS-47）
东营市渔业科技项目(20150217)资助 Supported by China Agriculture Research System (CARS-47) （20150217）
Fishery Science and Technology Project of Dongying City (20150217) （20150217）
